HiTrap™ MabSelect SuRe™ (Superior Resistance), HiTrap MabSelect™, and HiTrap MabSelect Xtra™ (Fig 1) are members of the HiTrap family of prepacked columns for purification of monoclonal antibodies (MAbs). Key performance characteristics of HiTrap MabSelect products include:

• Increased binding capacity compared with other protein A media
• High purity in one step
• Convenient, prepacked 1-ml and 5-ml columns
• Excellent for process development and screening of purification conditions
• Convenient operation with a syringe, a laboratory pump, or a chromatography system, such as ÄKTAdesign™

The protein-A ligand used in all three MabSelect media is produced by validated fermentation and downstream processes. The entire production process is free of components of mammalian origin. The resulting highly purified ligand is immobilized to the agarose matrix through a chemically stable thio-ether linkage.