GST MultiTrap™FF and GST MultiTrap 4B are prepacked disposable 96-well filter plates for reproducible high throughput screening of glutathione S-transferase (GST) tagged proteins. The
96-well filter plates are prepacked with Glutathione Sepharose™ 4 Fast Flow and Glutathione Sepharose 4B, respectively. GST-fusion proteins may be produced using the pGEX series of
expression vectors.

These filter plates simplify the purification screening and small scale purification of up to 0.5 mg of GST-tagged proteins/well. After thorough cell disruption, it is possible to apply unclarified
lysate directly to the wells in the 96-well filter plate without pre-centrifugation and/or filtration of the sample. It is recommended to extend the duration of mechanical/chemical lysis if the
sample is too viscous after lysis. The tagged proteins are eluted under mild, non-denaturing conditions that preserve protein antigenicity and function.