Dextrin Sepharose™ High Performance is a chromatography medium for purifying proteins tagged with maltose binding protein (MBP). Thanks to the high specificity of the binding, very high purity
is achieved in just one step. Recombinant proteins are engineered with MBP-tags to facilitate detection, isolation and purification procedures. In addition, the MBP-tag is often chosen due to its ability to increase the expression level and solubility of the fusion protein. Purification of MBP-tagged protein is done under physiological conditions, which together with mild elution by maltose, preserves the activity of the target protein.
Dextrin Sepharose High Performance is available in 25 and 100 ml lab packs and prepacked in 1 and 5-ml MBPTrap™ HP columns.