We have developed and optimized a capture step for the purification of a monoclonal antibody (MAb) on MabSelect SuRe™, which is a Protein A-based affinity chromatography medium. The use of PreDictor™ prefilled 96-well filter plates allowed us to investigate a large experimental space in order to find the best conditions for the capture step. Once these conditions were identified, fine tuning and verifications were carried out with HiScreen™ prepacked columns on an ÄKTA™ design system. Finally, a scale-up protocol was developed and tested under robust production conditions. The procedure described in this application note represents an efficient and robust solution for high throughput process development.