GE Healthcare Life Sciences Part of GE Healthcare

items:

total:

checkout

calculate my price

Hot list

Email page

Print page

Location: Home > Products > Gel and Blot Imaging & Analysis > Imaging Systems, Software, and Accessories > Storage Phosphor Screens and Cassettes

On-line Ordering
Catalog Home
Quick Order Form
Shopping Cart
Returns
Online ordering overview
Frequently Asked Questions

Product related documents

Last updated:09/02/2010

Storage Phosphor Screens and Cassettes

Requires approximately one-tenth the exposure time of traditional autoradiography film.
10 to 100 times more sensitive than film, depending on isotope and sample type.
Wide linear dynamic range (5 orders of magnitude) enables quantitation and visualization of both weak and strong signals in a single exposure.
Reusable screens do not require chemicals, darkroom, or other special treatment for use.
Each step can be performed at the lab bench under normal lighting conditions and at room temperature.
Results are digitized using a storage phosphor imaging system (e.g., Typhoon™, Storm™, or PhosphorImager™) and quantitated using ImageQuant™ software.

Page down for more information

^* For screen/system compatibility, please refer to the Selection Guide.
^† Contact your local sales representative for multiple screen pricing.

Storage Phosphor Screens and Cassettes

Technical Information

Click here to view this image in high resolution

Storage phosphor screens require less exposure time and are 10 to 100 times more sensitive than film.

Storage phosphor screens capture latent images produced by ionizing radiation (X-rays, b, and g emissions from isotopes such as ¹⁴C, ³H, ¹²⁵I, ¹³¹I, ³²P, ³³P, ³⁵S, etc.). Upon laser-induced stimulation, light is emitted from the storage phosphor screen in proportion to the amount of radioactivity in the sample. The resulting digital image allows for quantitation of subtle signal intensity differences over a wide dynamic range using storage phosphor imaging systems such as Typhoon™, Storm™, and PhosphorImager™. Typically, 50% to 90% less exposure time is required compared with an equivalent exposure to conventional film. Publication quality images are often obtained in a single exposure.

Storage phosphor screens are reusable and are not degraded by repeated exposure to laboratory levels of radioactivity. To reuse, simply expose a screen to the extra-bright light of ImageEraser light box.

Screen Styles
Mounted screens are permanently fixed to an aluminum backing plate. They require an exposure cassette. Older PhosphorImager™ Systems require mounted screens. Mounted screens are also compatible with Typhoon™ and Storm™ systems, but not with Typhoon FLA 9000 and Typhoon FLA 7000 imagers.

Unmounted screens have a flexible backing and are compatible only with Typhoon and Storm systems. They are available with standard autoradiography cassettes. Unmounted BAS screens (BAS-IP MS, BAS-IP SR and BAS-IP TR) are optimal for use on Typhoon FLA 9000 and Typhoon FLA 7000 imagers because of the magnetic backing layer, but they are also compatible with other Typhoon and Storm systems.

Screen Types

General Purpose (GP) and Multipurpose Standard (MS) Screens: GP and MS screens are reliable for a wide variety of applications and can be used with ³²P, ¹²⁵I, ³⁵S, ³³P, and ¹⁴C. The durable cellulose acetate coating and the phosphor layer formulation make the GP and MS screens ideal for ³²P and ¹²⁵I detection and quantitation. They are the screens of choice for ³²P Northerns and Southerns and ¹²⁵I Western blots and gels.

Tritium (TR) Screens: To detect the weak energy of the ³H signal, Tritium screens are constructed without a protective cellulose acetate overlay. The highly sensitive screens eliminate the need for fluorographic reagents. Best results are obtained when the ³H signal is on the surface of the sample and available to penetrate the screen.

Super Resolution (SR) Screens: SR screens are compatible with Typhoon FLA 9000 and Typhoon FLA 7000 systems. The screens provide higher resolution where a pixel resolution of 50 μm is required. For resolution of 100 μm or lower, the MS screens provide sufficient resolution.

SELECTION GUIDE BY INSTRUMENT COMPATIBILITY - Storage Phosphor Screens
Instrument	Type	Small Phosphor Screen 20 × 25 cm		Medium Phosphor Screen 20 × 40 cm		Large Phosphor Screen 35 × 43 cm		Half-size Screen 17.5 × 43 cm		Macroarray Phosphor Screen	Tritium Screen 19 × 24 cm
		Screen & Cassette	Screen only	Screen & Cassette	Screen only	Screen & Cassette	Screen only	Screen & Cassette	Screen only	Screen only	Screen & Cassette	Screen kit
PhosphorImager™ (small format) PSI, PSF	Mounted GP*	63-0034-89	63-0034-88			-	-	-	-	-	63-0035-50	63-0035-51 (1 mounted, 4 unmounted)
PhosphorImager™ (large format) 400, 425, 445	Mounted GP*	63-0034-89	63-0034-88			63-0034-82	63-0034-81	63-0034-85	-	-	63-0035-50	63-0035-51 (1 mounted, 4 unmounted)
Storm™ 820, 825, 830, 840, 845, 860, and 865	Mounted GP*	63-0034-89	63-0034-88			63-0034-82	63-0034-81	63-0034-85	-	-	63-0035-50	63-0035-51 (1 mounted, 4 unmounted)
	Unmounted GP*	63-0034-86	63-0034-87			63-0034-79	63-0034-80	63-0034-84	63-0034-83	63-0034-90	63-0035-49	-
Typhoon™ 8600, 8610, 9200, 9210, 9400, 9410, Trio, Trio⁺	Mounted GP*	63-0034-89	63-0034-88			63-0034-82	63-0034-81	63-0034-85	-	-	63-0035-50	63-0035-51 (1 mounted, 4 unmounted)
	Unmounted GP*	63-0034-86	63-0034-87			63-0034-79	63-0034-80	63-0034-84	63-0034-83	63-0034-90	63-0035-49	-
Typhoon FLA 7000	Unmounted MS^†	-	28-9564-75	-	28-9564-74	-	-
	Unmounted SR^‡	-	28-9564-78	-	28-9564-77	-	-
	Unmounted TR^§	-	28-9564-82	-	28-9564-81	-	-
Typhoon FLA 9000	Unmounted MS	-	28-9564-75	-	28-9564-74	-	28-9564-76
	Unmounted SR	-	28-9564-78	-	28-9564-77	-	-
	Unmounted TR	-	28-9564-82	-	28-9564-81	-	-
^*GP (General purpose screen): For use with ¹²⁵I, ³²P, ³³P, ³⁵S, and ¹⁴C ^† MS (Multipurpose standard screen): For use with ¹²⁵I, ³²P, ³³P, ³⁵S, and ¹⁴C. ^‡ SR (Super resolution screen): High resolution screens for use when a pixel resolution of 50 μm is required. ^§ TR (Tritium screen): High sensitivity screens for use with ³H.