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illustra TempliPhi DNA Sequencing Template Amplification Kit / illustra TempliPhi™ 100/500 Amplification Kit- Prepare circular DNA templates for cycle sequencing, cloning, and transformation in 4 to 6 h.
- Generate microgram quantities of template DNA from picogram amounts of starting material.
- Use amplified DNA directly for cycle sequencing without purification.
- Amplify DNA from bacterial or M13 liquid cultures, colonies, plaques, glycerol stocks, or purified circular (plasmid or M13) DNA.
- Simple protocol reduces time, labor, and consumables needed for template preparation, and workflow enables easy automation.
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illustra TempliPhi DNA Sequencing Template Amplification Kit / illustra TempliPhi™ 100/500 Amplification Kit
TempliPhi™ Kits use a novel process to efficiently prepare micrograms of circular DNA from picogram input material. The DNA templates are prepared by rolling circle amplification (RCA) using bacteriophage Phi29 DNA polymerase. TempliPhi™ uses an isothermal method for the exponential amplification of circular DNA. Phi29 DNA polymerase is active at 30°C, enabling amplification to be performed at this temperature without the need for thermal cycling. The TempliPhi™ protocol requires less than 20 min of hands-on time to amplify 96 samples from bacterial colonies.
Two different types of TempliPhi™ DNA Amplification Kits are available to meet different throughput requirements. illustra TempliPhi™ DNA Amplification Kit for 10 000 reactions contains premixed components and generates templates after overnight incubation. The 100- and 500-reaction kits contain separate, unmixed components for enhanced stability.
The illustra TempliPhi DNA Sequencing Template Amplification Kit has a distinct protocol and workflow compared to the 100/500 reaction size. Procedures for each kit are not interchangeable. Prior optimization is needed.
More information on Genomiphi can be found in our Templiphi web section.
Kinetics of DNA amplification using the illustra TempliPhi™ 100 Amplification Kit. Amplification of 1 ng pUC19 DNA over 24 h. The amount of DNA was quantitated using PicoGreen™ dsDNA Quantitation Reagents at the given times. Data is representative of triplicate experiment.
Components of each kit.
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Product related documents
Application note
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