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illustra NICK™ Columns- For rapid purification of labeled DNA (> 20 bases in length) from unincorporated radiolabeled nucleotides using gravity-flow chromatography.
- Prepacked with Sephadex™ G-50 DNA Grade (see illustra Sephadex™ G-50 and G-100 DNA Grade) in distilled water with 0.15% Kathon CG/ICP Biocide.
- Can accommodate sample volumes up to 100 μl.
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illustra NICK™ Columns
Technical Information
NICK™ Columns were originally designed for purification of nick-translated DNA fragments but are also suitable for separation of any labeled probe from unincorporated labeled nucleotides. They are ready for immediate use, and operate by gravity flow. Tests show that at least 97% of radioactivity applied in a nick-translated mixture elutes in two well-separated peaks, corresponding to labeled DNA and unincorporated nucleotides, respectively. At least 90% of the applied DNA is recovered. Maximum sample volume is 100 μl.
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