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Typhoon™ FLA 9000 Biomolecular Imager- High performance, flexible, and sensitive imaging for filmless autoradiography, fluorescence, chemifluorescence, 2-D DIGE, and gel documentation.
- Large 40 x 46 cm scan area for simultaneous imaging of up to two DIGE Gels or sixteen 10 x 8 cm blots facilitates comparisons, reducing workload and waiting time.
- Use multiple imaging modes at any time with no system modifications, reducing effort.
- 16-bit images at up to 10 μm pixel resolution give precise quantitation in gels, blots, tissue sections, and arrays.
- Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 32P, 33P, 35S, and other sources of ionizing radiation.
- Two- and three-color fluorescence scanning allows multiplex imaging (e.g., ECL Plex™ and 2-D DIGE), ensuring good quantitation. Flexible system can be adapted with stages, detectors, filters, and lasers to
optimize performance in new applications. Optional near infrared excitation for imaging IRDye™ and other infrared excited dyes.
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Typhoon™ FLA 9000 Biomolecular Imager
Technical Information
Typhoon FLA 9000 offers high performance for quantitating highly sensitive detection of proteins and nucleic acids with visible and IR excitation wavelengths.
Typhoon FLA 9000 is a variable mode laser scanner with modular access to the optical components, providing both versatile and flexible imaging for precise quantitation of proteins, nucleic acids, and other biomolecules.
The system provides several imaging modes at any time, including fluorescence and chemifluorescence, filmless autoradiography, and digitization of colorimetrically-stained samples (e.g., Coomassie™ blue and silver stain). It is also adequate for quantitative chemiluminescent imaging that does not require maximum sensitivity.
The system is optimized for 2-D DIGE imaging for differential protein expression studies, ECL™ Plus chemifluorescent imaging for quantitative protein detection by Western blotting, and multifluorescent ECL Plex imaging for precise quantitation of two or more proteins in the same blot.
The system accommodates four automated filters. Filters are easily accessed and exchanged without tools for attaining optimal imaging conditions.
Stages give the optimal imaging conditions for a range of samples. Samples include agarose and polyacrylamide gels, membranes, DIGE gels, radioisotope-labeled samples, as well as microplates and glass slides with the TP plug-in. The system can simultaneously scan two DIGE gels, each measuring up to 20 x 25 cm. The stages are easily removed from the system for cleaning.
A fast 1000 μm prescan function gives a rapid overview of the sample. The system provides a linear signal response over five orders of magnitude, providing a suitable basis for precise quantitation.
Lasers can be exchanged in the field to accommodate new applications and fluorophores.
| TECHNICAL SPECIFICATIONS |
| Detection modes | Fluorescence, chemifluorescence, filmless autoradiography, chemiluminescence, and digitization. |
| Excitation wavelengths | 473 nm (blue LD laser), 532 nm (green SHG laser), 635 nm (red LD laser), 685 nm (optional red LD laser), and 785 nm (optional near IR LD laser) |
| Radioisotopes | 3H, 14C, 32P, 33P, 35S, and 125I |
| Dynamic range | Five orders of magnitude |
| Bit depth | 16-bit |
| Max scanning area | 40 x 46 cm |
| Pixel sizes | 10, 25, 50, 100, 200 and prescan 1000 μm, selectable |
| Standard filters | IP (Phosphorimaging), LPB (510LP), LPG (575LP), LPR (665LP), BPB1 (530DF20), and BPG1 (570DF20) |
| Dimensions (W x D x H) | 900 x 800 x 400 mm |
| Weight | 97 kg |
| Line frequency | 50/60 Hz |
| Operating temperature | 15°C to 30°C |
| Humidity | 20% to 70% (no condensation) |
| Supply voltage | 100 - 200 VAC ± 10 % |
| Power consumption | Approx. 0.3 kVA |
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