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ImageQuant™ LAS 4000 Mini Biomolecular Imager- Exceptional sensitivity for quantitation of biomolecules labeled with chemiluminescent probes, and expandable to UV and blue fluorescence imaging. Supports gel documentation of Coomassie™ blue and silver-stained samples.
- Accurate quantitation of closely spaced bands in complex protein samples for gels and films up to 18 x 12 cm. Low noise for longer exposure times and lower background, important for precise quantitation of very weak signals.
- Wide linear dynamic range for precise quantitation of faint and dark bands over four orders of magnitude in the same image, avoiding time-consuming multiple film exposures.
- Distortion, dark frame, and flat frame corrections are applied to each imaging mode for optimal precision and uniform quantitation.
- Sensitive detection of less than 40 pg of protein on a Western blot. Sensitivity may be increased by "binning" of up to 8 x 8 pixels and smoothing.
- Chemiluminescent and
colorimetric signals can be captured in the same image without changing the lens. Upgradeable with UV transillumination for documenting EtBr-stained gels.
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ImageQuant™ LAS 4000 Mini Biomolecular Imager
Technical Information
ImageQuant LAS 4000 mini is a compact solution designed for sensitive digital imaging of Western, Northern, and Southern blots for the purposes of quantitation and documentation. The sensitivity is excellent for capturing chemiluminescent signals for quantitative detection purposes from Amersham™ ECL™, ECL Plus, and ECL Advance™ Western blotting detection reagents.
ImageQuant LAS 4000 mini is a compact and cost-effective solution designed for sensitive digital imaging of Western, Northern, and Southern blots for the purposes of quantitation and documentation. Compared to ImageQuant LAS 4000, it offers identical sensitivity for chemiluminescent Amersham™ ECL™ Western blotting reagents, as well as documentation of colorimetrically-stained gels (e.g., Coomassie blue and silver stain).
High resolution and precise quantitation of low signals are achieved using a 16-bit, 3.2 Mpixel camera specially developed for chemiluminescence imaging, fitted with a bright, wide aperture FUJINON™ F0.85 lens that projects sharp images onto a uniquely patterned CCD chip. By diagonally aligning octogonal pixels, the gap between pixels in the horizontal and vertical planes is reduced. This pattern allows an effective image resolution of 6.3 Mpixels.
The system consists of an intelligent dark box, camera, lens, and filter holder. Focusing, illuminators, and exposure time are remotely controlled by a computer. Simple operation allows adjustment without having to open the cabinet. The system can be upgraded to handle imaging applications requiring UV fluorescence, provided by a UV transilluminator for documentation of gels stained with ethidium bromide (EtBr). The intelligent dark box provides safe UV illumination.
The user-friendly control software performs several capture modes for achieving optimal sensitivity and dynamic range including increment, repetition, and program modes, with up to 8x8 binning. For image analysis, advanced and flexible ImageQuant TL software is available separately and comprises a dedicated suite of analysis applications to provide high levels of automation and accuracy in analysis of gels and blots.
| TECHNICAL SPECIFICATIONS |
| CCD model | Fujifilm™ Super CCD Area Type chip (15.6 x 23.4 mm) |
| Lens model | FUJINON Lens F0.85 43 mm |
| Cooling | Two-stage thermoelectric module with air circulation |
| Cooling temperature | -25°C; max. -30°C (at room temperatures below 28°C) |
| Dynamic range | 16-bit, four orders of magnitude |
| Chip resolution | 2048 x 1472, 3.2 Mpixels |
| Image resolution | Maximum 3072 x 2048, 6.3 Mpixels |
| Pixel size | Approximately 11 μm |
| Focusing and diaphragm | Automatic, remote operation |
| Capture modes | Automatic, manual (normal/incremental/repetitive/program) |
| Exposure time | Automatic, manual (1/100 s to 30 h) |
| Pixel correction | Dark frame correction, flat frame correction, distortion correction. |
| Image quality correction | Binning, smoothing |
| Binning modes | 1 x 1, 2 x 2, 4 x 4, 8 x 8 |
| Image Size | Up to 12 MB (.GEL and .TIF) |
| Sample size | 18 x 12 cm |
| Interface | USB 2.0 |
| Dimensions (W x H x D) | 440 × 750 × 380 mm |
| Weight | 40 kg |
| Line frequency | 50 / 60 Hz |
| Operating temperature | 15° C to 28° C |
| Operating humidity | 30% to 70% (no condensation) |
| Supply voltage | 100 to 240 V |
| Power consumption | Approx. 0.3 kVA |
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Product related documents
Application note
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