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Storm™- Large format imaging systems for filmless autoradiography, fluorescence, and chemifluorescence imaging.
- Filmless autoradiography based on proven PhosphorImager™ technology.
- Direct red- and blue-excited fluorescence enable rapid and accurate quantitation using fluorescence- and chemifluorescence-based methods.
- Five orders of linear dynamic range ensure accurate, publication-quality results on the first exposure.
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Storm™
Technical Information
Storm 865 Gel and Blot Imaging System offers filmless autoradiography, fluorescence, and chemifluorescence imaging.
Storm Gel and Blot Imaging System delivers proven PhosphorImager capability for autoradiography, direct fluorescence for nucleic acid and protein gel analysis, and chemifluorescence for fast blot analysis. Storm uses storage phosphor screens instead of film to deliver high-resolution imaging and accurate quantitation of 14C, 3H, 125I, 32P, 33P, 35S, and other sources of ionizing radiation.
The system’s wide exposure range and accurate signal quantification yield publication-quality images on the first exposure. Storage phosphor screens are reusable and are not degraded by repeated exposure to laboratory levels of radioactivity. To reuse, simply expose a screen to the extra-bright light of Image Eraser light box (included with Storm systems). With direct fluorescence, Storm enables visualization and analysis of nucleic acid and protein gels just minutes after electrophoresis is complete. Gels are soaked in dye solution and then placed in the scanner for analysis. Molecules labeled with Cy5 can also be directly detected.
For chemifluorescence-based methods, Storm reads blots in minutes, without exposure to film. Quantitation is simplified because, unlike film, Storm exhibits a linear response to fluorescent signal intensities.
Storm scans gels, blots, or mounted and unmounted storage phosphor screens up to 35 × 43 cm. All Storm systems come with ImageQuant™ TL.
Atlas™ Human cDNA array probed with 33P-labeled cDNA. The hybridized array, containing 588 unique targets, was exposed to a storage phosphor screen for 2 days and imaged using Storm 840.
Gene Discovery Array Human I probed with 32P-labeled human cDNA. The 22 × 22 cm membrane array contained 18 394 non-redundant cDNA clones. The array was exposed to a storage phosphor screen for one hour and imaged using Storm 820.
| TECHNICAL SPECIFICATIONS |
| Scan area | 35 × 43 cm |
| Sensitivity (limit of detection) | < 2 dpm/mm2 for 14C (200 and 100 μm only) using GP screen in a one-hour exposure. |
| Pixel size | 200, 100, and 50 μm, selectable. The scanning laser beam is approximately 50 μm in diameter. |
| Spatial resolution | Autoradiography: 2 line pairs/mm for lines drawn with 14C ink.
Blue-excited fluorescence, chemifluorescence: 2 line pairs/mm
Red-excited fluorescence: 4 line pairs/mm |
| Uniformity | ± 5% relative standard deviation |
| Pixel accuracy | ± 0.15% |
| Data format | 16-bit (65 536 levels), TIFF |
| Linear dynamic range and linearity | 5 orders of magnitude (100 000:1) with less than 7.5% relative standard deviation for entire dynamic range. |
| Light source | Storm 825, 845, and 865 use a red laser diode (635 nm). Storm™ 845 and 865 also use a blue LED (450 nm). |
| External interface | SCSI |
| Software | Scan control software for Windows™ operating system. |
| Power requirements | Storm: 115/230 V, 50-60 Hz, < 150 W
ImageEraser: 115/230 V, 50-60 Hz, < 150 W |
| Dimensions (W × D × H) | Storm: 76 × 76 × 38 cm
ImageEraser: 42 × 32 × 63.5 cm |
| Safety certifications | UL 3101, CAN/CSA C22.2 No 1010.1, EN 61010-1, EN60825-1, EN 61326, CE Marking (LVD 73/23/EEC EMC 89/336/EEC), CDRH (FDA) Subpart 21 CFR Ch.1-Part 1040. (Please contact your local GE Healthcare office for the latest list of certifications.) |
| SELECTION GUIDE - Storm System Capabilities |
| Storm Model | Storage phosphor
autoradiography | Direct red-excited
fluorescence | Direct blue-excited
fluorescence |
| 825 |  | | |
| 845 | | | |
| 865 | | | |
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