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Disposable PD-10 Desalting Columns- Fast, affordable, high-recovery desalting and buffer exchange, as well as removal of low-molecular weight compounds packed with Sephadex™ G-25 Medium.
- Flexible protocols allow runs by gravity flow or centrifugation.
- Easy to use and equilibrate; apply sample; elute.
- Preparation of the samples in the range of 1.0 to 2.5 ml by gravity flow and 1.75 to 2.5 ml with centrifugation.
- Desalt multiple samples in parallel with a desalting capacity >90%.
- Recovery in the range 70 to >95%.
- LabMate Buffer Reservoir makes it easier and more convenient to equilibrate PD-10 columns.
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Disposable PD-10 Desalting Columns
Technical Information
PD-10 columns are packaged with a convenient column stand¾the PD-10 Desalting Workmate. Use LabMate Buffer Reservoir for easier equilibration.
Disposable PD-10 Desalting Columns offer an inexpensive and convenient alternative to lengthy and troublesome dialysis procedures. In minutes, biological samples are desalted and small molecular weight solutes are removed or exchanged with excellent recoveries of macromolecules.
The instructions for Disposable PD-10 Desalting Columns have been updated with two new application protocols: gravity or centrifugation, to increase the flexibility of the product. The gravity protocol allows a simple cleanup of samples with one or several columns in parallel, without the need for a purification system. The spin protocol enables runs in parallel in a standard centrifuge with minimal dilution of the eluted sample.
Four adapters are included in each product pack to enable easy centrifugation. Together with LabMate PD-10 Buffer Reservoir, wash and equilibration buffer can be applied in one step with the gravity flow protocol (order separately).
Empty PD-10 columns are recommended for use with bulk media for affordable and versatile sample preparation.
| TECHNICAL SPECIFICATIONS |
| Disposable PD-10 Desalting Columns |
| Matrix | Sephadex G-25 Medium |
| Separation mechanism | According to size |
| Particle size range | 85-260 μm |
| Exclusion limit | Mr 5000 |
| Sample volume (gravity) | 1.0 to 2.5 ml |
| Sample volume (spin) | 1.75 to 2.5 ml |
| Column volume | 13.5 ml |
| Medium volume | 8.3 ml |
| Column material | Polypropylene barrel and polyethylene frits |
| Chemical stability | All commonly used buffers |
| Working pH range | 2¾13 |
| Storage temperature | 4°C to 30°C |
Removal of NaCl from BSA using the gravity protocol. Recovery of the sample was 95% and the desalting capacity was > 99%.
PD-10 Desalting Columns give fast desalting with gravity flow or spin protocols. The blue adapters are used together with the spin protocols.
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