 Autoradiography
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Amersham Hyperfilm ECL
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- Superior contrast and improved band visibility in your results.
- Publication-quality images¾the risk of visible artifacts (e.g. plastic wrap marks) is reduced due to the improved antistatic coating.
- Easier reading of images without compromising on sensitivity due to the clear, colorless base.
- Improved detection of lower concentration targets through longer exposure times without increasing the background.
- Greater confidence in results¾Hyperfilm ECL™ suitable for use with all blue and green light chemiluminescent systems and validated for use with Amersham ECL, Amersham ECL Plus, Amersham ECL Advance™ and CDP-Star™.
- Ease of use¾Amersham Hyperfilm ECL can be processed in automatic processors or manually using most common X-ray film developers and fixers.
- Applications¾chemiluminescent detection in Western, Southern, and Northern blots.
For performance comparisons, read more about
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Amersham Hyperfilm MP
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- Superior contrast and improved band visibility in your results.
- Publication-quality images¾the risk of visible artifacts (e.g. plastic wrap marks) is reduced due to the improved antistatic coating.
- Easier reading of images without compromising on sensitivity due to the clear, colorless base.
- Improved detection of lower concentration targets through longer exposure times without increasing the background.
- Increased flexibility¾Hyperfilm MP suitable for detection of 3H, 14C, 32P, 33P, 35S, 125I in direct or indirect autoradiography.
- Ease of use Amersham Hyperfilm MP is processed using automatic processors or manually using most common laboratory X-ray film developers and fixers.
For performance comparisons, read more about or
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Amersham Hyperfilm™ 3H
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- A speciality film with a single emulsion and no anti-scratch layer for the direct autoradiography of 3H.
- Ideal for whole body autoradiography, in situ hybridization, and receptor localization applications.
- Single-side format ensure maximum resolution.
- Clear base offers high contrast against blackened image for easy reading.
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Amersham Hyperscreen™ Intensifying Screens
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- Offer rapid results and high sensitivity for detecting 32P and 125I in Southern, Northern, and Western blots.
- Light emission from calcium tungstate phosphor is fully compatible with Hyperfilm™ MP.
- Use with Sensitize™ Pre-Flash Unit for even higher sensitivity and accurate quantitation.
- Fine grain screens give clear band resolution.
- Available in a full range of sizes to match Hypercassette™ and Hyperfilm sizes.
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Amersham Hypercassette™ Autoradiography Cassettes
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- Robust and chip-proof autoradiography cassettes for light-tight exposures at temperatures as low as -70°C.
- Maximum contact between film and sample.
- Nonporous pad gives a wipe clean surface ensuring minimum contamination.
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Amersham Amplify™ Fluorographic Reagent
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- Fast and convenient fluorographic reagent for 35S-, 14C- and 3H-labeled samples in gels.
- Increased detection efficiency 1000-fold for 3H and 15-fold for 35S and 14C.
- Less than two hours total preparation time.
- Can be used with acrylamide or agarose gels with no change in protocols.
- Odorless, inorganic and fully compatible with Sensitize™ Preflash unit and Hyperfilm™ MP.
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Amersham Hypertorch™
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- Red light darkroom torch for localized illumination.
- Minimizes film backgrounds by only illuminating local areas of work in a darkroom.
- Allows visibility in areas of darkroom where fixed safelights cannot reach.
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Amersham Hypercoat™ Nuclear Emulsions
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- Ready-to-use nuclear emulsions that can be used in the microautoradiography of radiolabels in tissues or cells.
- Uniform crystal size and aggregate-free emulsions ensure high resolution.
- Use Hypercoat LM-1 (0.2 μm crystal size) for general microautoradiography with 14C, 35S, 33P, 32P, 3H, and 125I.
- Use Hypercoat EM-1 (0.13 μm crystal size) for high-resolution light or electron microscopy with 3H-labeled samples.
- High silver content allows reduced exposure times.
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Amersham Microscale Autoradiography Standards
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Amersham Sensitize™ Preflash Unit
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- Specially designed flashgun for use with Hyperfilm™ ECL™ or Hyperfilm MP in conjunction with Hyperscreen™ Intensifying Screens to improve sensitivity and quantitation.
- Most effective when detecting small amounts of radioactivity or chemiluminescent signal.
- High quality orange filter included to ensure optimum wavelength for preflashing.
- Sealed unit to prevent white-light leakage.
- Calibration protocol included to ensure reproducibility.
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Amersham TrackerTape™
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- A phosphorescent tape which, when written on, can be used to label samples and corresponding autoradiographs.
- Produces a permanent negative image in minutes.
- Allows samples to be matched to results.
- Ordinary marker pen is used to write on the tape.
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Amersham Hyperprocessor™ Automatic Film Processor
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- An advanced automatic film processor of compact bench top design, requiring less than 0.2 m2 of floor space.
- Develops, fixes, washes, and dries film automatically in 2.5 min.
- Internal control systems ensure consistent high performance.
- Handles film sizes from 10 × 10 cm to 35 × 43 cm.
- Can process 70 films, size 24 × 30 cm, per h.
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