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HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs- Maximum recovery and minimum nonspecific adsorption with long-term chemical and physical stability resulting from the hydrophilic, rigid allyl dextran/ bisacrylamide matrix.
- Sephacryl™ S-100 HR is designed for separating peptides and small proteins.
- S-200 HR and S-300 HR are for purifying antibodies, serum proteins, and mid-size proteins.
- S-400 HR and S-500 HR are used to separate polysaccharides, macromolecules with extended structures, and even small plasmids.
- S-1000 SF is optimized for the purification of dextrans up to 108 molecular weight, spherical particles up to 400 nm, and DNA up to 20 000 base pairs including plasmids, vesicles, and viruses.
- Convenient, prepacked HiPrep™ Sephacryl™ columns speed up protein purification.
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HiPrep™ Sephacryl™ HR Columns and Sephacryl™ Lab Packs
Technical Information
HiPrep™ Sephacryl™ HR columns, prepacked with Sephacryl™ High Resolution media, provide reliable gel filtration separations.
HiPrep™ Sephacryl™ HR prepacked gel filtration columns offer excellent separation properties in a convenient, ready-to-use format. By combining the performance of Sephacryl™ High Resolution media with a low cost column construction, they are both reliable and cost effective to use with simple equipment or systems.
Five HiPrep™ Sephacryl™ HR columns are available prepacked with S-100, S-200, S-300, S-400, or S-500 HR media, respectively. Each column is also available in two sizes: 16/60 for sample volumes of up to 5 ml and 26/60 for sample volumes of up to 13 ml. The range of HiPrep™ Sephacryl™ HR prepacked columns allows purification of molecules with molecular weights as small as 1 × 103 to macromolecules as large as 2 × 107.
| TECHNICAL SPECIFICATIONS |
| Sephacryl™ High Resolution |
| Fractionation range (Mr) - | |
| - Globular proteins | |
S-100 HR
S-200 HR
S-300 HR
S-400 HR | 1 × 103 - 1 × 105
5 × 103 - 2.5 × 105
1 × 104 - 1.5 × 106
2 × 104 - 8 × 106 |
| - Dextrans | |
S-200 HR
S-300 HR
S-400 HR
S-500 HR
S-1000 HR | 1 × 103 - 8 × 104
2 × 103 - 4 × 105
1 × 104 - 2 × 106
4 × 104 - 2 × 107
5 × 105 - > 108 |
| Exclusion limit up to 400 nm for spherical particles. |
| - DNA exclusion limit | |
S-200 HR
S-300 HP
S-400 HR
S-500 HR
S-1000 HR | 30 bp
118 bp
271 bp
1078 bp
20 000 bp |
| Matrix | Spherical allyl dextran and N, N’-methylenebisacrylamide |
| Chemical stability | Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine-HCl, 1% SDS,
2 M NaCl, 24% ethanol,
30% propanol, 30% acetonitrile,
0.5 M NaOH (for cleaning) |
| pH stability | 3-11 (long term and working)
2-13 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
Physical stability
Autoclavability
Antimicrobial agent | Negligible volume variation due to changes in pH or ionic strength
At 121°C, pH 7 for 30 minutes (gel only)
20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
| Autoclavability | At 121°C, pH 7 for 30 minutes (gel only) |
| Antimicrobial agent | 20% ethanol |
Selectivity of the five different prepacked HiPrep Sephacryl™ HR columns.
| TECHNICAL SPECIFICATIONS |
| Prepacked HiPrep™ Sephacryl HR Columns |
| Bed dimensions | |
HiPrep™ 16/60
HiPrep™ 26/60 | 16 × 600 mm
26 × 600 mm |
| Bed volume | |
HiPrep™ 16/60
HiPrep™ 26/60 | 120 ml
320 ml |
| Sample volume | |
HiPrep™ 16/60
HiPrep™ 26/60 | < 5 ml
< 13 ml |
| Rec. linear flow rate | 5-15 cm/h |
| Max. linear flow rate | 30 cm/h |
| (H2O at 25°C) | |
| Max. pressure over the packed bed during operation | 1.5 bar (22 psi, 0.15 MPa) |
| Column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) |
| Theoretical plates | 5000 m-1 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
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