Webinar: New Approaches to Quantitative Western Blotting

This webinar was originally broadcast on November 27, 2009, and has been recorded so that you may view them at your convenience.

View the recorded webinar (requires registration/log in).

Speaker Åsa Hagner McWhirter, Ph.D. Application Research Scientist GE Healthcare Åsa Hagner McWhirter is currently an R&D application research scientist and coordinates the market support activities for the protein detection and analysis area at GE Healthcare in Uppsala Sweden. With over 5 years at GE Healthcare, she is a resident technical expert in fluorescent and chemiluminescent detection, as well as in the Ettan™ DIGE technology.

Abstract We will describe what is important for obtaining quantitative results using the ECL detection systems and how you can avoid the most common pitfalls. The technique has evolved to include fluorescent detection, which extends the dynamic range over the classic chemiluminescent approach. You’ll also learn how you can apply multiplexed approaches to Western blotting using fluorescent-labelled antibodies and a high performance fluorescent imager. Using two (duplex) or three (triplex) fluorescently labelled antibodies, you can improve quantitation and your Western blotting results.