ImageQuant LAS 4000 – the sensitive imager for multiple applications and users

ImageQuant LAS 4000 is a multipurpose CCD camera system for sensitive and quantitative imaging of blots by Amersham ECL chemiluminescence, with standard UV transillumination for EtBr gel documentation.

ImageQuant LAS 4000 can be upgraded with UV, IR and RGB fluorescence and white epi- and transillumination for documenting Coomassie Blue and silver stained gels. ImageQuant LAS 4010 provides UV and RGB epifluorescence preinstalled, as well as UV and white transillumination.

ImageQuant LAS 4000 – Features and benefits Highest resolution for reliable quantitation 16-bit 3.2 megapixel camera provides up to 6.3 megapixel virtual imaging Multiple capture modes make use of four orders of dynamic range Field corrections for imaging gels up to 25 x 25 cm Fast & sensitive Bright F0.85 lens designed for sensitive chemiluminescent imaging Up to 8x8 binning to detect weak signals Versatile and upgradeable Chemiluminescence and UV transmission is standard Epi-UV, visible & infrared fluorescence and white light can be added at any time Easy and convenient for multiple imaging applications Intuitive capture software Merges molecular marker image with chemiluminescent signal Easy exchange of accessories such as filters and light sources Hassle-free installation with a single cable Robust Stable platform with a low maintenance cost Suitable for a multiuser environment

Technical specification

CCD model	Fujifilm Super CCD Area Type chip (15.6 × 23.4 mm)
Lens model	FUJINON Lens F0.85 43 mm
Cooling	Two-stage thermoelectric module with air circulation
Cooling temperature	Down to -30°C (at room temp. below 28°C)
Dynamic range	16-bit, 4 orders of magnitude
Chip resolution	2048 × 1472, 3.2 Mpixels
Image resolution	Maximum 3072 × 2048, 6.3 Mpixels
Pixel size	Approximately 11 µm
Read pixel size	Down to 35 µm
Focusing and diaphragm	Automatic, remote operation
Capture modes	Automatic, manual (normal/incremental/repetitive/program)
Exposure time	Automatic, manual (1/100 s to 30 h)
Pixel correction	Dark frame correction, flat frame correction, distortion correction
Image quality correction	Binning, smoothing
Binning modes	1 × 1, 2 × 2, 4 × 4, 8 × 8
Image Size	Up to 12 MB (.GEL and .TIF)
Sample size	21 × 14 cm (25 × 25 cm with SIGMA wide view lens)
Interface	USB 2.0
Dimensions	510 × 900 × 480 mm
Weight	60 kg
Line frequency	50 to 60 Hz
Temperature	15°C to 28°C
Humidity	30% to 70% (no condensation)
Supply voltage	100 to 240 V
Power consumption	Approx. 0.3 kVA

Imaging Application

Ratings are based on overall system performance including model-specific features, versatility, and sensitivity (limit of detection).
Ratings are given for standard configurations. ImageQuant LAS 4000 can be upgraded to the same performance as ImageQuant LAS 4010. ImageQuant LAS 4000 mini can be upgraded with epi-UV and epi-blue fluorescence.

++++ Superior performance
+++ High performance
++ Good performance
+ Acceptable performance

Imaging Applications I	ImageQuant LAS 4010	ImageQuant LAS 4000	ImageQuant LAS 4000 mini
Chemiluminescence
Amersham ECL	++++	++++	++++
Amersham ECL Prime	++++	++++	++++
Amersham ECL Plus	++++	++++	++++
Amersham ECL Advance	++++	++++	++++
Fluorescence via UV transilluminator (312 nm)
EtBr	++++	++++	Optional
Deep Purple	+++	+++
Fluorescence via UV epi-illuminator (365 nm)
EtBr	++++	++++	Optional
SYBRO™ Rose	++++	++++
Qdot™ 605, 655, 705, 800	++	++
Fluorescence via blue epi-illuminator (460 nm)
AlexaTM Fluor 488	+++	Optional	Optional
Cy2	++
SYBRTM Green I & II	++++
SYBR Gold	++++
SYPRO Ruby	++++
SYPRO Orange	+++
FITC	++
FAM™	++
EGFP	++++
ECFP	+++
AttoPhos™	+++
Pro-QTM Emerald 488	++

++++ Superior performance +++ High performance ++ Good performance + Acceptable performance

Imaging Applications I	ImageQuant LAS 4010	ImageQuant LAS 4000	ImageQuant LAS 4000 mini
Fluorescence via green epi-illuminator (520 nm)
SYPRO Red	+++	Optional	Not available
Cy3	++
TAMRA™	+++
5-ROX™	++
HEX™	+++
Alexa Fluor 532, 546, 555	++
Deep Purple	+++
Pro-Q Diamond	++
BODIPYTM 576/589	++
R-phycoerythrin	+++
RFP	+++
HNPP	+++
Fluorescence via red epi-illuminator (630 nm)
Alexa Fluor 633, 635	+++	Optional	Not available
Alexa Fluor 647, 700	++
Cy5	++
BODIPY 650/665	+
DiD	++
TOTOTM 3	+++
DDAO Phosphate	+++
Fluorescence via IR epi-illuminator (710 nm)
Dy676	++
Alexa Fluor 700, 750	++
Gel documentation via white epi-illuminator/white transilluminator
Silver, CBB, NBT/BCIP, X-ray film	++	Optional	+

ImageQuant Imagers

Datafile

ImageQuant LAS 4000 biomolecular imager pdf