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Typhoon™ FLA 7000 – the fast, versatile scanner for multiple users
Typhoon FLA 7000 is a fast laser scanner optimized for quantitative phosphorimaging, ECL Plus Westerns, visible single fluorescence and gel documentation. High speed, full functionality and ease of use make the system an appropriate choice for multiuser facilities.
Typhoon FLA 7000 provides full visible fluorescence with 473/532/635/650 nm excitation and up to 25 µm resolution for gel and blot imaging.
Typhoon FLA 7000 - Features and benefits
High speed scanning – scans a 20 x 40 cm gel in 2.5 minutes.
- Less waiting and queues
- High speed without compromising sensitivity
16-bit image data captured at up to 25 μm resolution
- High resolution for accurate quantitation of closely spaced bands
Versatile range of applications: phosphorimaging, visible fluorescence, gel documentation
- Imaging modes are available at any time without user modification
Four preinstalled lasers
- Covers all the common applications in multiuser environments
Exchangeable filters in commercial standard format
- Easily adapted to new fluorophores 20 x 24 cm scan area
- Scans up to 8 blots measuring 8 x 10 cm for throughput and comparison
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Technical specification
| Detection modes | Fluorescence, chemifluorescence,
phosphorimaging, digitization |
| Excitation wavelengths | 473 nm (blue LD laser),
532 nm (green SHG laser),
635 nm (red LD laser),
650 nm (red LD laser |
| Radioisotopes | 3H, 14C, 32P, 33P, 35S sources |
| Dynamic range | 5 orders of magnitude |
| Bit depth | 16-bit |
| Max Scanning | Phosphorimaging 20 x 40 cm
Fluorescence and digitization 24 cm x 40 cm |
| Pixel sizes | 25, 50, 100, 200 µm |
| Standard filters | IP (Phosphorimaging),
Y520, O580, R670 |
| Dimensions (W × H ×D) | 940 x 556 x 360 mm |
| Weight | 62 kg |
| Line frequency | 50/60 Hz |
| Temperature | 15°C - 30°C |
| Humidity | 30% to 70% (no condensation) |
| Supply voltage | 100 - 240 VAC ± 10% |
| Power consumption | Approx. 0.3 kVA |
Imaging Application
Ratings are based on overall system performance including model-specific features, versatility, and sensitivity (limit of detection).
Blank fields indicate that data are not available.
++++ Superior performance
+++ High performance
++ Good performance
+ Acceptable performance
- Not applicable
 | Typhoon 9400/9410 | Typhoon FLA 9000 | Typhoon Trio/Tro+ | Typhoon FLA 7000 |
| Storage Phosphor | | | | |
| 32P, 125I, 14C, 35S, 33P, 3H | ++++ | ++++ | ++++ | ++++ |
| Macroarray (radiolabeled) | ++++ | ++++ | ++++ | - |
| Fluorescence—Proteins | | | | |
| CyDye DIGE Fluors | | | | |
| Cy2 | ++++ | +++ | ++++ | - |
| Cy3 | ++++ | ++++ | ++++ | - |
| Cy5 | ++++ | ++++ | ++++ | - |
| ECL Plex Fluors | | | | |
| Cy2 | ++++ | +++ | ++++ | - |
| Cy3 | ++++ | ++++ | ++++ | - |
| Cy5 | ++++ | ++++ | ++++ | - |
| Protein Stains | | | | |
| Deep Purple Total Protein Stain | ++++ | ++++ | ++++ | +++ |
| SYPRO Ruby | ++++ | ++++ | ++++ | ++++ |
| NanoOrange™ (solutions) | +++ | | +++ | |
| Pro-Q Diamond (phosphorylated proteins) | ++++ | +++ | ++++ | ++ |
| Pro-Q Sapphire 5 (Histidine-tagged proteins) | ++++ | +++ | ++++ | ++ |
| ELISA | | | | |
| AttoPhos | - | ++++ | - | +++ |
| Fluorescence—Nucleic acids | | | | |
| Cy3 and Cy5 | ++++ | ++++ | ++++ | +++ |
| Alexa Fluor 532 and Alexa Fluor 633 | ++++ | ++++ | ++++ | +++ |
| Nucleic acid stains | | | | |
| Ethidium Bromide (post stain) | ++++ | +++ | ++++ | +++ |
| Vistra Green, SYBR Gold, SYBR Green I & II | ++++ | ++++ | +++ | +++ |
| PicoGreen, RiboGreen | +++ | - | +++ | - |
| Chemifluoresence (enzyme-catalyzed) | | | | |
| ECL Plus Western blotting | ++++ | ++++ | ++++ | +++ |
| ECF, AlkPhos direct ECF | ++++ | +++ | ++++ | ++ |
| DDAO Phosphate | ++++ | ++++ | ++++ | +++ |
| Multipurpose fluorescence | | | | |
| Cy2 | ++++ | +++ | ++++ | ++ |
| Cy3 | ++++ | ++++ | ++++ | +++ |
| Cy5 | ++++ | ++++ | ++++ | +++ |
| Fluorescein, FAM, FITC, Alexa Fluor 488 | ++++ | ++++ | ++++ | +++ |
| TET, HEX, ROX, TAMRA | ++++ | +++ | ++++ | ++ |
| Other applications | | | | |
| GFP | +++ | +++ | +++ | ++ |
| Chemiluminescence | | | | |
| ECL | + | + | + | - |
| ECL Plus | + | + | + | - |
| ECL Advance | + | + | + | - |
Typhoon™
Data file
Typhoon FLA 7000 biomolecular imager pdf
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