This handbook describes both theoretical and practical aspects of the technique, the media available and how to select them, together with application examples and detailed instructions for the most commonly performed procedures. Practical information, with many tips and hints drawn from over 40 years of experience in chromatography purification, guides beginners and experts toward obtaining the best possible results from the latest chromatography media.

Chapter 5 focuses on RPC, a technique that provides the highest-resolution separations and is well suited for analytical work, for example, in combination with mass spectrometry for identification and characterization or when checking product purity. RPC can also be used as a final polishing step in a purification strategy, but care must be taken to ensure that the presence of organic solvents does not compromise recovery of biological activity or tertiary structure.