GE Healthcare Life Sciences - Basic principles

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Education Centre
Education Centre

Purpose of purification
Developing purification protocols
Purification development - summary
How to combine purification steps
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Hydrophobic interaction chromatography
Animation
Basic principles
The Separation Mechanism
Elution modes
The HIC experiment
Effect of choice of ligand
Effect of eluent composition
Effect of temperature
Resolution in HIC
Optimisation of HIC experiments
HIC in Practice
Technique Profile
What is HIC?

Basic principles in Hydrophobic interaction chromatography

Contents

1.	The Separation Mechanism
		- Definitions
		- Hydrophobic interaction
		- Adsorption/Desorption
		- Desorption curves
2.	Elution modes
		- Gradient elution
		- Step elution
3.	The typical HIC experiment
4.	Effect of choice of ligand
5.	Effect of eluent composition
		- Salt
		- Running pH
6.	Effect of temperature
7.	Resolution in HIC
8.	Optimisation of HIC experiments