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Education Centre
Education Centre
Purpose of purification
Developing purification protocols
Purification development - summary
How to combine purification steps
LC techniques
Affinity Chromatography
Desalting & Gel Filtration
Hydrophobic interaction chromatography
Starting a session
Selecting protein
In the lab
Ammonium sulphate percipitation
Chromatography
Electrophoresis
Progress report
Purification completed
Your virtual supervisor
Thank you

Chromatography

This is the main screen of the UNICORN™ software that you use to control the ÄKTA™ system. In the dropdown menu you can select to work with Gel filtration, Hydrophobic Interaction, Affinity Chromatography or Ion exchange. Each method requires you to enter some parameters. Those parameters are method specific and on this screenshot the user has selected and entered the parameters for, Ion Exchange.

Analyse the sample with electrophoresis.