GE Healthcare Life Sciences - Estimation of protein molecular weights by SDS gel electrophoresis

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Location: Electrophoresis > Applications > SDS PAGE > Estimation of protein molecular weights by SDS gel electrophoresis

Applications
1D Protein Analysis
SDS PAGE
SDS gel electrophoresis
Estimation of protein molecular weights by SDS gel electrophoresis
Materials and equipment
Procedure
Native gel electrophoresis
Preparing linear gradient gels
Separating proteins by flatbed SDS-PAGE
Separating Proteins on denaturing mini-gels
Related files
IEF Separation
Detection: Western Blotting
Detection: Gel Staining
Imaging
2D Protein Analysis
Sample Preparation
1st Dimension IEF
2nd Dimension SDS PAGE
Detection
Imaging
Troubleshooting
Ettan DIGE system
Ettan DIGE system
Fragment Analysis
Principles
Applications
Separation
Detection and Imaging

Introduction

Estimating the size or molecular weight of a protein is relatively straight-forward with SDS gels. The general procedure is to separate a set of standards (proteins with known molecular weights) in parallel with the unknown or sample protein. The standards are used to generate a curve correlating molecular weight and migration in the gel, from which the molecular weight of the unknown sample can be determined. The gel concentration should be chosen so that the standards produce a linear curve in the region of the unknown.