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 Location: Electrophoresis > Applications > SDS PAGE > SDS gel electrophoresis > Materials and equipment
Applications
1D Protein Analysis
SDS PAGE
SDS gel electrophoresis
Stock Solutions
Materials and equipment
Procedure
Troubleshooting
References
Estimation of protein molecular weights by SDS gel electrophoresis
Native gel electrophoresis
Preparing linear gradient gels
Separating proteins by flatbed SDS-PAGE
Separating Proteins on denaturing mini-gels
Related files
IEF Separation
Detection: Western Blotting
Detection: Gel Staining
Imaging
2D Protein Analysis
Sample Preparation
1st Dimension IEF
2nd Dimension SDS PAGE
Detection
Imaging
Troubleshooting
Ettan DIGE system
Ettan DIGE system
Fragment Analysis
Principles
Applications
Separation
Detection and Imaging

Materials and equipment

SE 600 vertical slab gel unit with casting stand1.5- or 0.75-mm-thick combs and spacers
1.5- or 0.75-mm-thick combs and spacers Glass Plates
50 ml and 125 ml side-arm flasksTransfer pipettes
Pipettor capable of pipetting 1–20 µl with long, narrow "gel-loading" pipette tipsWater aspirator or vacuum pump with trap
Boiling-water bath or 100 ºC temperature blockEPS 301 Power Supply
Magnetic stirrerPipettes/graduated cylinders


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