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Location: Home > Applications > 2-D Electrophoresis > Sample Preparation > CyDye DIGE Fluor Labeling Reagents

2-D Electrophoresis
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Sample Preparation
Tissue / Cell Disruption
Contaminant Removal / Inactivation
Contaminant Removal by Affinity Chromatography
Contaminant Removal by IEX
Contaminant Removal by HIC
Fractionation
CyDye DIGE Fluor Labeling Reagents
CyDye DIGE Fluor, Minimal Dye
CyDye DIGE Fluor for Scarce Samples
CyDye DIGE Fluor for Scarce Samples and Preparative Gels
1st Dimension - IEF
2nd Dimension - SDS
Gel Staining
Detection
Image Analysis
Spot Processing for MS Analysis

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CyDye DIGE fluor labeling reagents

This step is needed when performing DIGE applications

For DIGE work pH the buffer using Tris (minimal pH8.5, saturation 8). For saturation labeling use TCEP as the reductant. Triton is not recomended for DIGE work. Always use Urea and Thiorea in combination for DIGE applications.