 Ion Exchange Chromatography
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SELECTION GUIDE - Ion Exchange
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SELECTION GUIDE BY TECHNICAL OVERVIEW - Ion Exchange Chromatography
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SELECTION GUIDE-Overview of HiTrap, HiPrep, HiLoad Prepacked Columns for IEX
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MonoBeads™ Columns
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- Innovative, monodisperse 10-μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
- MonoBeads™ available in a choice of three prepacked column formats¾Tricorn™, HR, and PC columns.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- Compatible with ÄKTAdesign™, FPLC™ System, and other high-performance LC systems.
- MonoBeads™ are also available as Mono P for chromatofocusing (see Mono P™ Columns).
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MiniBeads™ Columns
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- Innovative,monodisperse 3-μm nonporous beads yield exceptional resolution, speed, reproducibility, and durability with somewhat lower capacity than MonoBeads™.
- Tricorn™ high-performance columns (4.6/50 PE) prepacked with MiniBeads™ are optimized for use with ÄKTAdesign and comparable LC systems.
- Nonporous matrix of MiniBeads™ improves resolution and flow kinetics, better withstands back pressure, and minimizes nonspecific adsorption of the sample.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- PC columns are designed for micropurification.
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RESOURCE Q
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- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/ml, and improved stability in organic solvents and pH extremes.
- RESOURCE columns are prepacked with SOURCE 15Q or SOURCE 15S for fast separation. Separation times using 1 ml RESOURCE columns are less than 3 min at 9.6 ml/min and about 20 min using a peristaltic pump at 1 ml/min.
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SOURCE™ Media/RESOURCE™ and Tricorn Columns for IEX
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- SOURCE 15Q and 15S media are ideal for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/ml, and improved stability in organic solvents and pH extremes.
- SOURCE 15Q 4.6/100 PE and SOURCE 15S 4.6/100 PE are Tricorn high-performance columns designed for high-resolution purification at lab scale and for optimization studies when scaling up.
- RESOURCE columns are prepacked with SOURCE 15Q or SOURCE 15S for fast separation. Separation times using 1 ml RESOURCE columns are less than 3 min at 9.6 ml/min and about 20 min using a peristaltic pump at 1 ml/min.
- SOURCE 30Q and 30S media are designed for intermediate purification and large-scale polishing.
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Capto IEX Media/HiTrap Capto IEX Columns
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- High-throughput medium for capture and intermediate purification at process-scale.
- Combined high binding capacity, high flow rate, and low backpressure properties reduce process cycle times and improve productivity.
- Rigid agarose matrix enables high bed heights and purification of viscous samples at high flow rates.
- Flow rates of up to 700 cm/h can be employed at process-scale with a backpressure below 3 bar.
- HiTrap™ prepacked column format for easy screening and convenient process development.
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Capto MMC Media/HiTrap Capto MMC
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- High dynamic binding capacity at high conductivity.
- High volume throughput.
- New selectivity allows binding in the presence of salt.
- Prepacked HiTrap™ columns for easy screening and convenient process development.
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Capto adhere/HiTrap Capto adhere
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- Contaminant removal to formulation levels in post protein A purification
- Prepacked HiTrap columns for easy screening and convenient process development.
- High capacity and productivity in flow-through mode
- Wide operational window of pH and conductivity
- Savings in time and operating costs with a two-step chromatographic process
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HiTrap Capto IEX Selection Kit
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- Fast and easy selection with five different ion-exchange ligands on Capto.
- Excellent for media screening: includes five 1 ml HiTrap columns prepacked with Capto Q, Capto S, Capto DEAE, Capto MMC, Capto adhere, connectors, and instructions.
- Excellent for optimization studies: buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
- Designed for use with syringe, peristaltic pump and other chromatography systems such as ÄKTAdesign.
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HiScreen Capto IEX Columns
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- Prepacked ready to use columns with different Capto IEX media for convenient process development optimization
- Ideal for parameter method optimization and parameter screening due to the 10 cm bed height
- Easy connection in series to achieve 20 cm bed height
- Small bed volume gives fast results and minimal sample/buffer consumption
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity
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HiTrap™ IEX FF Columns
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- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), and ANX Sepharose™ 4 Fast Flow (high sub) (weak anion).
- Designed for use with syringe, peristaltic pump, or chromatography systems such as ÄKTA™design.
- High flow rates, and good scale-up potential.
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HiTrap™ IEX Selection Kit
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- Fast and easy selection with seven different ion-exchange ligands on Sepharose™ Fast Flow and Sepharose™ XL, prepacked in 1-ml HiTrap™ columns.
- Excellent for optimization studies: buffer composition, pH, flow rate, sample loading, and elution scheme can be optimized with small sample quantities prior to scale-up.
- Excellent for screening different media: includes seven 1-ml HiTrap™ columns prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), ANX Sepharose™ 4 Fast Flow (high sub) (weak anion), Q Sepharose™ XL (strong anion), and SP Sepharose™ XL (strong cation), connectors, and instructions.
- Designed for use with syringe, peristaltic pump and other chromatography systems such as ÄKTA™design.
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HiScreen™ IEX FF Columns
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- Prepacked ready-to-use columns with Q Sepharose Fast Flow and SP Sepharose Fast Flow for convenient process development optimization.
- Ideal for parameter method optimization and parameter screening due to the 10 cm bed height.
- Easily connected in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
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HiPrep™ IEX FF 16/10 Columns
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- Q, SP, DEAE, and CM Sepharose™ 6 Fast Flow in prepacked 20-ml HiPrep™ columns provide fast, reliable, and reproducible preparative ion exchange separations.
- Ideal for rapid enrichments during initial capture of proteins from starting material.
- Simple operation.
- Compatible with single-pump configurations and other chromatography systems such as ÄKTA™design.
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IEX Sepharose™ Fast Flow
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- For preparative purifications, even when purifying crude samples.
- Available with strong (Q and SP) and weak (DEAE, CM, and ANX) anion and cation exchangers, in bulk quantities and in prepacked HiTrap™ and HiPrep™ columns, as well as HiTrap™ IEX Selection Kit.
- For separation of proteins of all pI values with Q and SP having a high dynamic capacity for proteins over the entire pH range.
- ANX has a different selectivity compared to DEAE, and is applicable for separation of high molecular weight proteins.
- Easy scale-up from HiTrap™ scale to laboratory scale to process scale.
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HiTrap™ IEX XL Columns
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- Prepacked with either Q Sepharose™ XL (strong anion) or SP Sepharose™ XL (strong cation).
- Designed for use with a syringe, peristaltic pump or chromatography system such as ÄKTA™design.
- For group separations, concentrations, and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- High binding capacities with high flow rates.
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HiPrep™ IEX XL Columns and IEX Sepharose™ XL Lab Packs
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- Prepacked with Q Sepharose™ XL* or SP Sepharose XL media, 20-ml preparative anion- and cation-exchange columns provide high loading capacity and fast purifications.
- Optimized for reliable, reproducible separations.
- Q Sepharose XL virus licensed is specifically intended for the purification of viruses.
- Compatible with simple pump-based configurations and other chromatography systems such as ÄKTA™design.
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HiTrap™ IEX HP Columns
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- Prepacked with Q Sepharose™ High Performance or SP Sepharose™ High Performance for high resolution purifications on 34-μm beads.
- Convenient and reproducible for fast, easy, high-performance separations either alone or connected in series.
- Designed for use with a syringe, peristaltic pump and chromatography system such as ÄKTA™design.
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HiScreen™ IEX HP Columns
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- Prepacked ready-to-use columns with Q Sepharose High Performance and SP Sepharose High Performance for convenient process development optimization.
- Ideal for parameter method optimization and parameter screening due to the 10 cm bed height.
- Easy connection in series to achieve 20 cm bed height.
- Small bed volume gives fast results and minimal sample/buffer consumption.
- Reproducible results, scalable to BioProcess columns packed with the same media using the same linear fluid velocity.
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HiLoad™ IEX HP Columns and IEX Sepharose™ High Performance Lab Packs
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- High-performance preparative separations where high efficiency and high resolution are desired.
- Highly cross-linked agarose matrix provides excellent chemical and physical stability.
- Available as strong anion exchangers (Q Sepharose HP) and strong cation exchangers (SP Sepharose HP), analogous to Mono Q™ and S, and Q and SP Sepharose™ Fast Flow.
- Easy to use. For optimal performance and reproducibility, Sepharose™ High Performance is also available in prepacked HiTrap™ columns.
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Mini Q PC 3.2/3
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- Innovative,monodisperse 3 μm nonporous beads yield exceptional resolution, speed, reproducibility, and durability with somewhat lower capacity than MonoBeads™.
- Tricorn™ high-performance columns (4.6/50 PE) prepacked with MiniBeads™ are optimized for use with ÄKTAdesign and comparable LC systems.
- Nonporous matrix of MiniBeads™ improves resolution and flow kinetics, better withstands back pressure, and minimizes nonspecific adsorption of the sample.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- PC columns are designed specifically for SMART™ System.
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Mini S PC 3.2/3
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- Innovative,monodisperse 3 μm nonporous beads yield exceptional resolution, speed, reproducibility, and durability with somewhat lower capacity than MonoBeads™.
- Tricorn™ high-performance columns (4.6/50 PE) prepacked with MiniBeads™ are optimized for use with ÄKTAdesign and comparable LC systems.
- Nonporous matrix of MiniBeads™ improves resolution and flow kinetics, better withstands back pressure, and minimizes nonspecific adsorption of the sample.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- PC columns are designed specifically for SMART™ System.
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Mono Q PC 1.6/5
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- Innovative, monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
- MonoBeads available in a choice of three prepacked column formats¾Tricorn, HR, and PC columns.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- Compatible with ÄKTA design, FPLC System, and other high-performance LC systems.
- MonoBeads are also available as Mono P for chromatofocusing (see Mono P™ Columns).
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Mono S PC 1.6/5
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- Innovative, monodisperse 10 μm porous beads yield high resolution, dynamic capacity, reproducibility, and durability.
- MonoBeads available in a choice of three prepacked column formats¾Tricorn, HR, and PC columns.
- Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
- Compatible with ÄKTA design, FPLC System, and other high-performance LC systems.
- MonoBeads are also available as Mono P for chromatofocusing (see Mono P™ Columns).
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HiTrap Q Sepharose Fast Flow
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- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Designed for use with syringe, peristaltic pump, ÄKTA™design system and FPLC™ System.
- High flow rates, and excellent scale-up potential.
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HiTrap CM Sepharose Fast Flow
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- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Designed for use with syringe, peristaltic pump, ÄKTA™design system and FPLC™ System.
- High flow rates, and excellent scale-up potential.
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HiTrap DEAE Sepharose Fast Flow
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- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Designed for use with syringe, peristaltic pump, ÄKTA™design system and FPLC™ System.
- High flow rates, and excellent scale-up potential.
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HiTrap ANX Sepharose 4 Fast Flow (high sub)
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- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Designed for use with syringe, peristaltic pump, ÄKTA™design system and FPLC™ System.
- High flow rates, and excellent scale-up potential.
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HiTrap Q Sepharose XL
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- Designed for use with a syringe, peristaltic pump, FPLC™ System, or ÄKTA™design systems.
- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- High binding capacities with high flow rates.
- Excellent for scaling up.
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HiTrap SP Sepharose XL
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- Designed for use with a syringe, peristaltic pump, FPLC™ System, or ÄKTA™design systems.
- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- High binding capacities with high flow rates.
- Excellent for scaling up.
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HiTrap SP FF
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- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Prepacked with SP Sepharose™ Fast Flow (strong cation).
- Designed for use with syringe, peristaltic pump, ÄKTA™design system and FPLC™ System.
- High flow rates, and excellent scale-up potential.
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MacroCap SP
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- For purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (PEGylated proteins) intended for use as biopharmaceuticals.
- Highly porous matrix provides excellent mass transfer of sample and large surface area for maximized adsorption of biomolecules.
- Allows separation of mono- from oligo- and non-PEGylated proteins with high selectivity under high load conditions.
- Good cleaning-in-place (CIP) stability for long medium lifetime and excellent process economy.
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