 Introduction to Amersham CyDye™ Fluors
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- Versatile fluorophores for use in a broad range of applications.
- pH tolerance (pH 3-10) allows use at biologically relevant pH.
- DMSO tolerance enables transfer from storage to assay without loss of performance.
- Good aqueous solubility eliminates need for organic solvents in assay buffers.
- Superior photostability (compared with other dyes, e.g., fluorescein) improves performance.
- High purity and thorough QC testing ensures high levels of chromophore and reactive dye content.
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Amersham CyDye™ Reactive Dye 5-Packs
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- Convenient way to label antibodies, proteins, drugs, and oligonucleotides with CyDye™ fluorescent dyes.
- For use in ELISA, immunoprecipitation, and blotting applications.
- Dyes are packaged in premixed amounts and foil sealed to ensure consistent labelings.
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Amersham CyDye™ Fluors for Protein Arrays
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Amersham CyDye™ Value Packs (mono-Reactive NHS Ester)
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- Targeted and specific labeling of amine residues on antibodies, peptides, or oligonucleotides.
- Bulk packs available for greatest economy.
- Example protocols included with each dye pack.
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Amersham CyDye™ Value Packs (bis-Reactive NHS Ester)
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- Suitable for general labeling of amine residues.
- Large range of bulk packs available for greatest economy.
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Cy3 and Cy5 Maleimide mono-Reactive Dye 5-packs
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- More selective method of antibody labeling that reduces the possibility of the label interfering with subsequent antibody-antigen reactions.
- Provides an alternative for when CyDye™ NHS esters are unsuitable.
- Dyes are packaged in premeasured amounts and foil-sealed to ensure consistent labelings.
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Amersham CyDye™ Value Packs (mono-Reactive Maleimide)
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- For the selective labeling of free sulfhydryl groups such as cysteine residues in peptides, proteins, and oligonucleotides.
- Particularly suitable for the labeling of antibodies.
- Large range of bulk packs available for greatest economy.
- Example protocols included with each dye pack.
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Amersham CyDye™ Value Packs (mono-Reactive Hydrazide)
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- For the labeling of free carbonyl groups of glycoproteins and carbohydrates.
- Range of bulk packs available for greatest economy.
- Example protocols included with each dye pack.
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Select-a-Dye mono-Reactive Dye Packs
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- Enables a variety of labeling strategies using NHS ester, maleimide, or hydrazide to be investigated.
- Available as either Cy3 or Cy5.
- Example protocols included with each dye pack.
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Amersham CyDye™ Antibody Labeling Kits
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- Convenient way to tag antibodies with CyDye™ fluorescent dyes.
- Conjugations are carried out under mild conditions and are complete within 1 h.
- Dyes are packaged in premeasured amounts and foil sealed to ensure consistent labelings.
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