|
|
MacroCap SP- For purification of large biomolecules such as polyethylene glycol (PEG)-modified proteins (PEGylated proteins) intended for use as biopharmaceuticals.
- Highly porous matrix provides excellent mass transfer of sample and large surface area for maximized adsorption of biomolecules.
- Allows separation of mono- from oligo- and non-PEGylated proteins with high selectivity under high load conditions.
- Good cleaning-in-place (CIP) stability for long medium lifetime and excellent process economy.
Page down for more information
MacroCap SP
Technical Information
MacroCap™ SP cation exchanger can be used to purify PEGylated and other large biomolecules to high purity and yield at high sample loads. Robust chemical stability helps ensure a long medium lifetime.
MacroCap™ SP is a cation exchanger designed to purify PEGylated and other large biomolecules, at high sample load. The base matrix is highly porous, which gives high available surface area for adsorption of large molecules. Mono-PEGylated proteins can be separated to high purity from oligo- and nonPEGylated proteins in with high selectivity and high sample load in a single run. The large pore size also makes MacroCap™ SP suitable for binding other large proteins.
The excellent chemical stability of MacroCap™ SP allows cleaning-in-place to be performed both in acidic and alkaline conditions. The hydrophilic nature of the MacroCap™ SP base matrix reduces nonspecific binding and reduces fouling issues that may be experienced with more hydrophobic base matrices. Together, these features assure long medium lifetime.
| TECHNICAL SPECIFICATIONS |
| Matrix | Cross-linked copolymer of allyl dextran and N,N-methylene bisacrylamide |
| Media type | Strong cation exchanger, SO3- |
| Particle size* | 50 μm (d50V) |
| Total ionic capacity | 0.10-0.13 mmol H+/ml medium |
| Flow rate | 120 cm/h in BPG 300, 20-cm bed height or 70 cm/h in BPG 300, 30-cm bed height using process buffers with the same viscosity as water at < 3 bar (0.3 MPa) |
| Recommended separation range | a) Proteins in excess of Mr 150 000
b) Functionalized dextrans or PEGs greater than or equal to Mr 20 000
PEGylated proteins containing greater than or equal to Mr 10 000 of PEG (total) per conjugate. |
| pH stability | |
| short term | 2-13 |
| working | 3-12 |
| long term | 4-11 |
| CIP stability (short term) | 2-13 |
| Storage | 0.2 M sodium acetate in 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Chemical stability | All commonly used aqueous buffers, 0.5 M NaOH, 0.1 M citric acid, 25% ethanol, 30% propanol, 30% methanol, 50% ethylene glycol, 1% Tween 20, and 1% SDS |
| * d50v is the medium particle size of the cumulative volume distribution.
|
For technical information, please download the instruction and data file in the Product Related Documents list on the left.
|
|
Product related documents
Application note
BioProcess media. Process-scale quantities are available.)
