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Exonuclease I- Elimination of residual single-stranded DNA containing a 3'-terminus.
- Measuring endonucleolytic cleavage of covalently closed circular (ccc) ssDNA.
- Measuring DNA helicase activity.
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Exonuclease I
Technical Information
Source: E. coli strain containing an overproducing clone of E. coli Exonuclease I.
Description: Acts specifically on single-stranded DNA degrading it processively in the 3'- to 5'-direction producing 5'-mononucleotides.
Unit definition: One unit catalyzes the release of 10 nmol of acid-soluble nucleotide from denatured DNA in 30 min at 37°C under standard conditions.
Purity: Free of contaminating endonucleases, double-stranded exonucleases and ribonucleases. Greater than 95% pure as determined by SDS-PAGE.
Activity: 10 units/μl.
Storage conditions: 20 mM Tris-HCl, pH 7.5, 0.5 mM EDTA,
5 mM 2-mercaptoethanol, and 50% glycerol. Store at -20°C.
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