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Shrimp Alkaline Phosphatase (E.C.3.1.3.1)- Removes 5'-phosphates from DNA and RNA.
- Easily inactivated by heat.
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Shrimp Alkaline Phosphatase (E.C.3.1.3.1)
Technical Information
Source: Arctic shrimp (Pandalus borealis).
Description: A high specific activity, heat-labile alkaline phosphatase.
Unit definition: One unit catalyzes the hydrolysis of 1 μmol of
p-nitrophenyl phosphate per minute at pH 10.4 (glycine/NaOH buffer) at 37°C.
Purity: The enzyme is purified to apparent homogeneity and is free of all contaminating endonucleases, exonucleases and ribonucleases.
Activity: 1 unit/μl.
Storage conditions: 25 mM Tris-HCl, pH 7.6, 1 mM MgCl2,
0.1 mM ZnCl2 and 50% glycerol. Store at -20°C.
Note: Completely and irreversibly inactivated in Tris buffers at
pH 8.0-8.5 by heating for 15 min at 65°C. No further treatment is necessary.
Functional testing: Dephosphorylation of a restriction enzyme digested plasmid (5-20 pmol of 5'-ends, 0.1-0.5 units/pmol 5'-ends). Reduces re-ligation to < 0.5% compared to the untreated control.
Supplied with 10× reaction buffer: 200 mM Tris-HCl, pH 8.0,
100 mM MgCl2.
Supplied with dilution buffer: 50 mM Tris-HCl, pH 8.0.
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