|
|
Nucleon™ BACC Genomic DNA Extraction Kits- Developed for rapid extraction of high quality, high molecular weight genomic DNA from blood and cell cultures.
- Features a safe, phenol-free protocol requiring only 30 min to complete.
- Available for three extraction scales - Nucleon™ BACC1 for 50 preps of 1 ml of whole blood or cultured cells (1 to 3 x 106 cells/ml). - Nucleon BACC2 for 50 preps of 10 ml of whole blood or cultured cells (3 to 10 × 106 cells/10 ml) 100 preps of 1 ml of whole blood or cultured cells (1 to 3 x 106 cells/ml). Note: Additional Reagent A will need to be prepared to process all 50 x 10 ml preps. - Nucleon BACC3 for 50 preps of 10 ml of whole blood (kit contains all reagents necessary for 50 x 10 ml blood extractions).
Page down for more information
Nucleon™ BACC Genomic DNA Extraction Kits
Technical Information
PCR amplification of blood and cell culture DNA purified using Nucleon™ BACC. Lane 1: 100 bp ladder, lane 2: negative template control, lanes 3-5: human blood DNA, lanes 6-8: HeLa cell DNA. PCR primers: Mouse methyltransferase gene. Separation on a 1.5% agarose gel.
| Principle steps in the Nucleon™ BACC protocol |
| cell lysis |
 |
| deproteinisation with sodium perchlorate |
|
| extraction with chloroform and Nucleon™ resin |
|
| DNA recovery |
|
| DNA washing |
Click here for more information about Nucleic Acid Purification/APTRIX/upp01077.nsf/Content/nucleic_acid_purification
|
|
Product related documents
Brochure
)
