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Nucleon™ PhytoPure™ Genomic DNA Extraction Kits- PhytoPure™ resin binds plant polysaccharides ensuring high yields of high-quality plant genomic DNA in only one hour.
- Two-kit sizes provide economical scale-up using the same phenol-free protocol.
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Nucleon™ PhytoPure™ Genomic DNA Extraction Kits
Technical Information
RAPD analysis of 4 species using operon primer OPA 10. DNA diluted to approximately 25 ng per lane and run on a 1.2% agarose gel in TBE. Lane 1: 1 kb ladder; lane 2: willow DNA extracted by Nucleon™ PhytoPure™; lane 3: willow DNA extracted by SDS/Phenol; lane 4: maize DNA extracted by Nucleon™ PhytoPure™; lane 5: maize DNA extracted by SDS/Phenol; lane 6: rye DNA extracted by Nucleon™ PhytoPure™; lane 7: rye DNA extracted by SDS/Phenol; lane 8: rhododendron DNA extracted by Nucleon™ PhytoPure™; lane 9: rhododendron DNA extracted by SDS/Phenol; lane 10: 1 kb ladder. Note the complete failure of the amplification reactions with willow DNA extracted by the SDS/phenol.
| PLANT SPECIES - PhytoPure™ successfully removed DNA |
| Arabidopsis thaliana | Lycopersicon esculentum |
| Brassica oleracea | Musa spp |
| Brassica napus | Malus spp |
| Capsicum annuum | Nicotiana tabacum |
| Capsicum frutescens | Phaseolus vulgaris |
| Cereals (barley, maize, rye, wheat) | Pinus sylvestris |
| Cocos nucifera | Pisum sativum |
| Helianthus annus | Rhododendron spp |
| Helianthus tuberosus | Salix spp |
| Hevea braziliensis | Solanum tuberosum |
| Irvingia gabonensis | Swietenia macrophylla |
| Lotus japonicus | Sphagnum, bog moss |
| Principle steps in the Nucleon™ Phytopure protocol |
| breaking of cell wall |
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| cell lysis with potassium SDS |
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| extraction with Nucleon™ Phytopure resin and chloroform |
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| DNA precipitation |
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| DNA washing |
Polysaccharide binding mechanism.
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