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MegaBACE™ SNuPe™ Genotyping Kit- MegaBACE™ SNuPe™ Genotyping Kit combines single-base extension technology with Thermo Sequenase™ DNA polymerase and standard rhodamine dyes.
- Convenient one-tube reaction premix format and easy-to-use injection marker for multiple injections increases throughput and reduces gel matrix consumption.
- MegaBACE™ SNP Profiler v1.0 software provides automated allele calling, editing, and verification functions.
- SNP analysis based on two parameters¾allele signal intensity and relative allele mobility.
- Quality scores assigned to each genotype for faster and easier review of automatically processed data.
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MegaBACE™ SNuPe™ Genotyping Kit
Technical Information
Multiple injection of SNuPe™ reactions on MegaBACE™ DNA Analysis System. Three SNuPe™ reactions were injected into two different capillaries. illusta AutoSeq™ 96 was used for post-reaction cleanup.
MegaBACE™ SNuPe™ Genotyping Kit enables four-color SNP genotyping of PCR products with MegaBACE™ DNA Analysis System. The kit contains Thermo Sequenase™ DNA polymerase and four dye-labeled dideoxynucleotides (terminators) in a single-tube premix format.
SNP genotyping on the MegaBACE™ takes advantage of the instrument's flexibility to load twelve, 96-well sample plates over a period of approximately 30 min, using a proprietary method of repeated, time-spaced injections. A multiple injection marker is added to each sample prior to injection, acting as an internal control and as a demarcation of one injection from the next. A total of 1200 SNP genotypes can be obtained with a turnaround time of less than 2 h.
MegaBACE™ SNP Profiler v1.0 is a Windows™ based software that automatically processes signal data and outputs SNP genotypes. Allele calling is based on the allele signal intensity in each channel and allele mobility in the specific injection. The software enables project management ability. Multiple runs of the same SNP or multiple injection run data can be analyzed at the same time.
The SNP Profiler main window displaying data for a selected marker. The allele table appears in the bottom left area of the screen, listing the allele calls for each injection from each well position. The summary plots at the top right display the allele distribution based on signal intensity and mobility (size), respectively. The traces showing allele peaks appear at the bottom right of the screen.
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Product related documents
Brochure
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, respectively. The traces showing allele peaks appear at the bottom right of the screen.'))
