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ExoSAP-IT- For rapid and efficient purification of PCR products.
- Features two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase, for the removal of unwanted deoxynucleotides and primers with no interference in downstream applications.
- One-tube, one-step method with no sample loss.
- Purified DNA is ready for immediate use in manual and automated sequencing.
- Eliminates spin columns to decrease time and expense to purify PCR product.
- Simple processing makes ExoSAP-IT ideal in robotics, replacing beads, filtrations, and plates.
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ExoSAP-IT
Technical Information
Schematic diagram of the ExoSAP-IT method.
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References
- Dugan, K. A. et al. J. Forensic Sci 47, 811-818 (2002).
- Hanke, M. and Wink, M. BioTechniques 17, 858-860 (1994).
- Mu, J. et al. Nature 418, 323-326 (2002).
- Silva, Jr., W. A. et al. BioTechniques 30, 537-542 (2001).
- Werle, E. et al. Nucleic Acids Res. 22, 4354-4355 (1994).
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