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Frequently Asked Questions
Last updated:07/31/2010

ExoSAP-IT

  • For rapid and efficient purification of PCR products.
  • Features two hydrolytic enzymes, Exonuclease I and Shrimp Alkaline Phosphatase, for the removal of unwanted deoxynucleotides and primers with no interference in downstream applications.
  • One-tube, one-step method with no sample loss.
  • Purified DNA is ready for immediate use in manual and automated sequencing.
  • Eliminates spin columns to decrease time and expense to purify PCR product.
  • Simple processing makes ExoSAP-IT ideal in robotics, replacing beads, filtrations, and plates.
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Store at -15 to -30°C.

ExoSAP-IT

Technical Information


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Schematic diagram of the ExoSAP-IT method.

* For licensing information, see Legal Info.

References

  1. Dugan, K. A. et al. J. Forensic Sci 47, 811-818 (2002).
  2. Hanke, M. and Wink, M. BioTechniques 17, 858-860 (1994).
  3. Mu, J. et al. Nature 418, 323-326 (2002).
  4. Silva, Jr., W. A. et al. BioTechniques 30, 537-542 (2001).
  5. Werle, E. et al. Nucleic Acids Res. 22, 4354-4355 (1994).

Click here for more information about Nucleic Acid Purification



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