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T7Sequencing Kit- For rapid generation of a kilobase or more of sequence information per template.
- Features an easy-to-dilute, uniform concentration of T7 DNA polymerase, sequencing mixes optimized to work with both single- and double-stranded templates and primer concentrations suitable for double-stranded sequencing.
- Includes a quick-denaturation protocol for double-stranded templates.
- Produces sequencing ladders with even band intensities that can be read unambiguously.
- Same mixes can be used with 32P- or 35S-labeled dATP or dCTP.
- Includes two sets of termination mixes: “Read Short” (0-500 nucleotides from the primer) and “Read Long” (1000 nucleotides or more, starting 10 to 50 bases from the primer).
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T7Sequencing Kit
Technical Information
T7Sequencing Kit has been formulated to take advantage of the properties of T7 DNA Polymerase: its high rate of synthesis for rapid generation of long chain-terminated products; its tolerance for substrate analogs; its ability to produce sequencing ladders with even band intensities; and its ability to read through difficult regions of secondary structure (1). This makes the enzyme a good choice for sequencing double-stranded DNA.
The kit contains the following reagents in sufficient quantities for performing 100 sequencing reactions: A, C, G and T mixes -short and long, labeling mix-dATP, labeling mix-dCTP, T7 DNA polymerase, stop solution, enzyme dilution buffer, annealing buffer, control template, universal primer, and instruction booklet.
* For licensing information, see Legal Info.
Typical results using T7Sequencing Kit and M13mp18 (+) template. The order of the lanes is G A T C. Numbers in margin are distances in base pairs from primer.
References
- Kristensen, T. et al., Nucl. Acids Res. 16, 3487 (1988).
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Product related documents
Instruction/protocol template. The order of the lanes is G A T C. Numbers in margin are distances in base pairs from primer.'))
