|
|
High Purity Solution dNTPs- Easy-to-use, time-saving solutions for applications requiring dNTPs of high purity such as amplification, dideoxy sequencing, labeling, mutagenesis, cDNA synthesis, and expression profiling.
- High Purity Solution dNTPs are functionally tested in long PCR* to be PCR-qualified, and meet or exceed criteria for high-quality sequencing with Thermo Sequenase DNA polymerase.
- High purity: > 98% triphosphate, as confirmed by chromatographic analysis and 31P nuclear magnetic resonance (NMR) spectroscopy.
- Supplied as buffer-free, ready-to-use solutions.
- Solution dNTPs are stable for 24 months when stored at -20 °C.
- Available either as individual products, in convenient sets, or premixes.
- dNTPs are also available as solids for your convenience.
Page down for more information
High Purity Solution dNTPs
Technical Information
As of April 30th 2007, these high purity solutions dNTPs will be discontinued and replaced with the improved illustra Solutions dNTPs for improved performance and ease of use.
For information concerning molecular weights, structures and extinction coefficients of representative nucleotides, see LINK:73912.
* For licensing information, see Legal Info.
High-purity nucleotides are qualified for long-range PCR. 100 ng of Lambda DNA was PCR amplified with two primers 20.7 kb apart. PCR was performed under standard conditions using mixes (containing three dNTPs) spiked with the fourth nucleotide (indicated above each lane). The PCR products were separated on a 0.8% agarose gel.
|
|
Product related documents
Application note
 spiked with the fourth nucleotide (indicated above each lane). The PCR products were separated on a 0.8% agarose gel.'))
