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illustra AutoSeq™ G-50- For rapid removal of unincorporated fluorescent dye-terminators from cycle sequencing reactions prior to automated sequencing.
- Columns are prepacked with Sephadex™ G-50 DNA Grade F and pre-equilibrated in double-distilled water with 0.05% Kathon CG/ICP Biocide added as a preservative.
- Columns that are ready to use enable sample application to collection of purified product in less than 4 min.
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illustra AutoSeq™ G-50
Technical Information
illustra™ AutoSeq™ G-50 columns are specifically designed to remove fluorescent dye-terminators from sequencing reactions prior to analysis on automated sequencers. Effective purification is essential for high-quality sequencing results since residual dye-terminators can obscure data.
illustra AutoSeq™ G-50 consists of MicroSpin™ columns containing Sephadex™ G-50, pre-equilibrated in double-distilled water. This is important because even the small amount of salt in traditional buffers (such as TE buffer) can cause electrophoretic artifacts on salt-sensitive automated sequencers.
illustra AutoSeq G-50 columns can be used to clean up reactions prepared with the BigDye Terminator v3.1 Cycle Sequencing Kit from Applied Biosystems .
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Product related documents
Application note
