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illustra GFX™ PCR DNA and Gel Band Purification Kit- For the isolation and concentration of DNA fragments (50 bp to 40 kb) from PCR mixtures, DNA-containing agarose gel bands, enzyme-based DNA modifications, and restriction digestions.
- Fast and easy to use method with less than 10 min hands-on time.
- Flexible 10 to 50 μl elution volume for different DNA concentration needs.
- Improved column design with increased maximum volume holding of up to 800 μl liquid volume (950 μl without a cap)
- Highly pure DNA is ready for direct use in sequencing, PCR, labeling, restriction enzyme digestion, and cloning.
- Addition of a visual color indicator to the capture buffer to ensure optimal pH for maximum DNA binding and recovery
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illustra GFX™ PCR DNA and Gel Band Purification Kit
Technical Information
The illustra™ GFX™ PCR DNA and Gel Band Purification Kit is designed for the rapid purification and concentration of PCR products or DNA fragments ranging in size from 50 bp to 10 kb. This kit can be used to purify DNA from reaction volumes up to 100 μl or agarose gel slices up to 900 mg. The kit combines a versatile chaotropic buffer with a glass fibre matrix supported in a spin-column for the purification of DNA from both solution and agarose gel. Typical recoveries range from 60 to 80% for DNA fragments from agarose gel to as high as 95% for PCR products from solution. DNA purity is exceptional; 99.5% of contaminants are removed.
The illustra GFX PCR DNA and Gel Band Purification Kit contains the following components in sufficient quantities: GFX columns, collection tubes and color-coded bottles of capture buffer, wash buffer, and two elution buffers (Tris-HCl and sterile water).
A 910 bp PCR fragment (from the Tumor Protein P53 ORF) was purified using the illustra GFX PCR DNA and Gel Band Purification Kit. Both PCR and agarose gel band extractions are shown for the same fragment, for both 50 μl and 10 μl elution volumes. A260/280 readings were used to determine purity levels.
A 910 bp PCR fragment (from the Tumor Protein P53 ORF) was purified using the illustra GFX PCR DNA and Gel Band Purification Kit. Both PCR and agarose gel band extractions are shown for the same fragment, for both 50 μl and 10 μl elution volumes. A260 readings were used to determine purity levels.
pH indicator
(A). A Yellow or pale orange color indicates that the mixture of capture buffer and sample is at an optimal pH for efficient binding of DNA to the silica membrane;
(B). A dark pink or red color shows that the pH of the mixture of capture buffer and sample is too high for efficient DNA adsorption to the silica membrane.
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Product related documents
Brochure
 was purified using the illustra GFX PCR DNA and Gel Band Purification Kit. Both PCR and agarose gel band extractions are shown for the same fragment, for both 50 %b5l and 10 %b5l elution volumes. A<sub>260/280</sub> readings were used to determine purity levels.'))
 was purified using the illustra GFX PCR DNA and Gel Band Purification Kit. Both PCR and agarose gel band extractions are shown for the same fragment, for both 50<UNspace/>%b5l and 10<UNspace/>%b5l elution volumes. A<sub>260</sub> readings were used to determine purity levels.'))
. A Yellow or pale orange color indicates that the mixture of capture buffer and sample is at an optimal pH for efficient binding of DNA to the silica membrane;(B). A dark pink or red color shows that the pH of the mixture of capture buffer and sample is too high for efficient DNA adsorption to the silica membrane.'))
