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GFX™ Genomic Blood DNA Purification Kit- For rapid and efficient purification of genomic DNA from whole blood, buffy coat, bone marrow, nucleated red blood cells, cultured cells, lymphocytes and buccal cells. With additional reagents, Gram-positive and -negative bacteria and yeast can also be used. Isolate DNA in as little as 15-20 min with a microcentrifuge to yield genomic DNA with a functional purity equivalent to DNA isolated using standard methods.
- Columns prepacked with a glass fibre matrix provide consistent results and reduce handling time.
- Eliminates the need for organic solvents and hazardous materials typically used in most genomic DNA isolation methods.
- Produces high-quality genomic DNA, ready for immediate use in applications such as PCR, automated and manual DNA sequencing, restriction enzyme analysis and Southern blotting.
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GFX™ Genomic Blood DNA Purification Kit
Technical Information
As of July 2007, this product will be discontinued and replaced with the illustra blood genomicPrep Mini Spin Kit for improved performance and ease of use.
Genomic DNA isolated from various sources using GFX™ Genomic Blood DNA Purification Kit. For each sample, DNA was eluted using 200 μl of TE buffer (pH 8.0). Each lane contains 250 ng of DNA as determined by absorbance at 260 nm. Whole blood-direct = 100 μl human whole blood processed using the Direct Method; whole blood scalable = 300 μl of human whole blood processed using the Scalable Method; chicken blood = 2.5 μl of chicken blood processed using the Direct Method; HeLa = 1 × 106 cells; COS = 2 × 106 cells; 3T3 = 2 × 106 cells. Cultured cells were processed using the procedure for Cultured Cells and Lymphocytes. M = l DNA-Hind III Digest (27-4048-01).
Triplicate 10 μl, 25 μl, and 50 μl samples of human whole blood were processed using the direct method of the GFX™ Genomic Blood DNA Purification Kit. Purified genomic DNA was eluted using 130 μl of TE buffer (pH 8.0). One-tenth of each purified sample was loaded onto an agarose gel. The volume of blood that was processed is indicated above the corresponding lanes of the gel. M = molecular weight marker.
GFX™ Genomic Blood DNA Purification Kit is designed for the rapid and efficient purification of high-quality genomic DNA from 10 to 1000 μl of fresh, frozen whole blood or from bone marrow. The Cultured Cells and Lymphocyte procedure can process up to 5 × 106 cultured cells or up to 1 × 107 lymphocytes. The kit is ideally suited for applications requiring up to 15 μg of genomic DNA. The method combines an optimized extraction/capture buffer with a spin-column prepacked with a glass fibre matrix to yield a quick and time-saving procedure for the isolation of high-quality genomic DNA.
GFX™ Genomic Blood DNA Purification Kit contains the following components in sufficient quantities for 100 purifications: RBC Lysis Solution, Extraction Solution, Wash Solution, GFX™ Columns, Collection Tubes and instruction booklet.
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Application note
. Each lane contains 250 ng of DNA as determined by absorbance at 260 nm. Whole blood-direct = 100 %b5l human whole blood processed using the Direct Method; whole blood scalable = 300 %b5l of human whole blood processed using the Scalable Method; chicken blood = 2.5 %b5l of chicken blood processed using the Direct Method; HeLa = 1 %d7 10<sup>6</sup> cells; COS = 2 %d7 10<sup>6</sup> cells; 3T3 = 2 %d7 10<sup>6</sup> cells. Cultured cells were processed using the procedure for Cultured Cells and Lymphocytes.<LineBreak/>M = <lamda/> DNA-Hind III Digest (27-4048-01).'))
. One-tenth of each purified sample was loaded onto an agarose gel. The volume of blood that was processed is indicated above the corresponding lanes of the gel.<LineBreak/>M = molecular weight marker.'))
