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Typhoon Trio™- Versatile system handles gel sandwiches, agarose and polyacrylamide gels, membranes, microplates, and even microarrays.
- Powerful excitation sources and innovative high-quality confocal optics allow for the sensitive detection of low-abundance targets.
- Red-, green-, and blue-excitation wavelengths and a wide choice of emission filters enable imaging of an extensive variety of fluorophores.
- Automated four-color fluorescence scanning allows multiplexing of multiple targets in the same sample ensuring accuracy of analysis, increasing throughput, and saving time.
- Storage phosphor technology delivers high-resolution imaging and accurate quantitation of 3H, 14C, 125I, 32P, 33P, 35S, and other sources of ionizing radiation.
- Highly sensitive optics enable direct chemiluminescent imaging without intermediate exposure to films or screens.
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Typhoon Trio™
Technical Information
Typhoon 9410 high performance gel and blot imager that can also image microarrays.
Typhoon™ 9410 Variable Mode Imager unites proven storage phosphor autoradiography technology with four-color, nonradioactive fluorescent labeling techniques. For DNA, RNA, and protein samples, choose from:
- Storage phosphor autoradiography
- Direct blue-excited fluorescence (457, 488 nm)
- Direct green-excited fluorescence (532 nm)
- Direct red-excited fluorescence (633 nm)
- Chemiluminescence
When one of the scanning modes is selected, the appropriate optical components are automatically activated. Typhoon scans gels, blots, and mounted or unmounted storage phosphor screens up to 35 × 43 cm. The instrument exhibits outstanding linearity, quantitative accuracy, and extremely low limits of detection.
Choose from four different Typhoon models depending on your imaging needs. For added flexibilty, Typhoon 9200, 9210, and 9400 can be upgraded as imaging needs change. All Typhoon models come with ImageQuant™ TL (see ImageQuant™ TL for details).
| TECHNICAL SPECIFICATIONS |
| Scan area | 35 × 43 cm |
| Sensitivity (limit of detection) | Storage Phosphor: 14C (1 h exposure, 200 and 100 µm only): < 2 dpm/mm2; 32P: 5-10-fold lower than 14C
488 nm excited fluorescence: 100 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
532 nm excited fluorescence: 200 amol HEX, TMR, ROX, and 400 amol FAM end-labeled DNA primer in 12% polyacrylamide gel sandwich, 0.4 mm thick
633 nm excited fluorescence: 200 amol Cy5 end-labeled DNA primer in 0.4 mm thick, 12% polyacrylamide gel with TBE buffer. |
| Pixel size | 1000, 500, 200, 100, 50, 25, and 10 µm, selectable. |
| Spatial resolution | Autoradiography: 2 line pairs/mm for lines drawn with 14C ink.
Blue-excited fluorescence: 10 line pairs/mm
Green-excited fluorescence: 10 line pairs/mm
Red-excited fluorescence: 10 line pairs/mm |
| Uniformity | ± 5% over entire scan area. |
| Pixel accuracy | ± 0.15% |
| Data format | 16-bit (65 536 levels), TIFF |
| Linear dynamic range and linearity | Five orders of magnitude (100 000:1) with less than 7.5% relative standard deviation for entire dynamic range. |
| Red light source | Type: 10 mW Helium-Neon laser Estimated average lifetime: ~ 10 000 h Wavelength: 632.8 nm |
| Green light source | Type: 20 mW solid-state doubled frequency SYAG laser Estimated average lifetime: ~ 10 000 h Wavelength: 532 nm |
| Blue light source | Type: 30 mW all blue lines Argon ion laser Estimated average lifetime: ~ 5000 h Wavelength: 488 nm (20 mW) and 457 nm (4 mW) |
| External interface | 10 Base-T Ethernet using TCP/IP protocol |
| Software | Scan control software for Windows™ operating system. |
| Power requirements | 115/230 V (switchable), 50-60 Hz, < 500 W |
Dimensions
(W × D × H) | Instrument: 118 × 78 × 48 cm
Blue laser module: 30 × 78 × 48 cm |
| Safety certifications | UL 3101, CAN/CSA C22.2 No 1010.1, EN 61010-1, EN60825-1, EN 61326, CE Marking (LVD 73/23/EEC EMC 89/336/EEC), CDRH (FDA) Subpart 21 CFR Ch.1-Part 1040. (Please contact your local GE Healthcare office for the latest list of certifications.) |
A microarray slide, spotted with cDNA from Microarray ScoreCard™ control plate and cDNA from Incyte Genomics, Inc. was hybridized with 25 pmol of Cy3-labeled skeletal muscle mRNA and Cy5-labeled liver mRNA. The slide was scanned on Typhoon 9410 at 10 µm pixel size resolution.
Two-dimensional 2-D protein gel stained with Deep Purple™ Total Protein Stain. Protein sample consists of HBL100 breast cell line and BT474 breast cell carcinoma. First dimension: Immobiline™ DryStrip pH 3-10 NL, 24 cm run on Ettan™ IPGphor IEF System. Second dimension: DALT Gel 12.5 run on Ettan DALTtwelve Large Vertical Electrophoresis System. Scanning: Typhoon 9400 Variable Mode Imager: excitation, green laser (532 nm); emission 560LP filter, PMT 530 V, 100 µm resolution. The gel image shows the pH 3-8 region, where most proteins are present.
Four-color competitive gel shift assay to determine the relative affinities of the Salmonella spp. bacteriophage P22 repressor Mnt (1). In the assay the repressor is allowed to bind four different oligonucloetides labeled with FAM, HEX, TAMRA and ROX respectively at the same time. The intensities for each fluorophore in a lane are related directly to the relative affinity of the repressor protein for the oligonucleotide used.
In-lane sizing of PCR products. GeneScan -500 ROX size standard (yellow, Applied Biosystems), Cy3-labeled PCR products (green), Alfexpress Cy5 Sizer™ 50-500 (red, GE Healthcare) and fluorescein-
labeled PCR products (blue) were
resolved in a 12% polyacrylamide
gel glass sandwich.
References
1. Tsz-Kwong, M. and Stormo, G. D. Nucleic Acids Res. 29, 2471-2478 (2001)
| SELECTION GUIDE - Typhoon™ Model Capabilities |
| Typhoon™ model | Storage phosphor
autoradiography | Chemiluminescence | Direct red-excited
fluorescence | Direct green-excited
fluorescence | Direct blue-excited fluorescence | Microarray imaging
capability |
| | | | | | 457 nm | 488 nm | |
| 9200 |  | | | | - | - | - |
| 9210 | | | | | - | - | |
| Trio | | | | | - | | - |
| Trio+ | | | | | - | | |
| 9400 | | | | | | | - |
| 9410 | | | | | | | |
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Product related documents
Application note
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; emission 560LP filter, PMT 530 V, 100 %b5m resolution. The gel image shows the pH 3<ENdash/>8 region, where most proteins are present.'))
. In the assay the repressor is allowed to bind four different oligonucloetides labeled with FAM, HEX, TAMRA and ROX respectively at the same time. The intensities for each fluorophore in a lane are related directly to the relative affinity of the repressor protein for the oligonucleotide used.'))
, Cy3<NonBRHyph/>labeled PCR products (green), Alfexpress Cy5 Sizer<TM/> 50<ENdash/>500 (red, GE<UNspace/>Healthcare) and fluorescein<NonBRHyph/><para/>labeled PCR products (blue) were<para/>resolved in a 12% polyacrylamide<para/>gel glass sandwich.'))
