| TECHNICAL SPECIFICATIONS |
| Superdex™ |
| Exclusion limit (Mr) |
| Superdex™ 75 | 1 × 105 globular protein |
| Superdex™ 200 | 1.3 × 106 globular protein |
| Separation range (Mr) |
| Superdex™ 75 | 3000-70 000 globular protein |
| Superdex™ 200 | 10 000-600 000 globular protein |
| Matrix | Spherical composite of cross-linked agarose and dextran |
| Average particle size | 13 μm |
| Chemical stability | Stable in all common buffers:
1 M acetic acid, 8 M urea,
6 M guanidine hydrochloride,
30% isopropanol, 70% ethanol,
1 M NaOH (for cleaning in place) |
| pH stability | 3-12 (working and long term), 1-14 (short term) |
| Superdex™ 10/300 GL Columns (Tricorn)* |
| Bed dimensions | 10 × 300 mm |
| Recommended sample volume | 25-250 μl |
| Bed volume | 24 ml |
| Max. pressure | |
| Superdex™ 75 | 18 bar (261 psi, 1.8 MPa) |
| Superdex™ 200 | 15 bar (217 psi, 1.5 MPa) |
| Max. flow rate (H2O at 25°C) |
| Superdex™ 75 | 1.5 ml/min |
| Superdex™ 200 | 1.0 ml/min |
| Theoretical plates | > 30 000 m-1 |
| Superdex™ PC 3.2/30 Columns |
| Bed dimensions | 3.2 × 300 mm |
| Bed volume | 2.4 ml |
| Recommended sample volume | 2-25 μl |
| Max. pressure | |
| Superdex™ 75 | 24 bar (348 psi, 2.4 MPa) |
| Superdex™ 200 | 12 bar (174 psi, 1.2 MPa) |
| Max. flow rate (H2O at 25°C) |
| Superdex™ 75 | 0.100 ml/min |
| Superdex™ 200 | 0.100 ml/min |
| Theoretical Plates | > 30 000 m-1 |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| * Columns are not suitable for use with ÄKTAprime™ plus system.
|
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