HiTrap™ IEX FF Columns- For method scouting, group separations, concentrations and sample cleanup.
- Convenient and affordable for fast, easy separations either alone or connected in series.
- Prepacked with Q Sepharose™ Fast Flow (strong anion), SP Sepharose™ Fast Flow (strong cation), CM Sepharose™ Fast Flow (weak cation), DEAE Sepharose™ Fast Flow (weak anion), and ANX Sepharose™ 4 Fast Flow (high sub) (weak anion).
- Designed for use with syringe, peristaltic pump, or chromatography systems such as ÄKTA™design.
- High flow rates, and good scale-up potential.
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HiTrap™ IEX FF Columns
Technical Information
Q, SP, DEAE, and CM Sepharose™ Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading capacities.
ANX Sepharose™ 4 Fast Flow (high sub) is based on 4% highly cross-linked beaded agarose. This results in a medium with higher porosity, which is particularly useful for the purification of high molecular mass proteins.
The active end of the charged group is the same for DEAE Sepharose™ Fast Flow and ANX Sepharose™ Fast Flow (high sub), the difference is the length of the carbon chain of the charged group. DEAE Sepharose™ Fast Flow has a diethylaminoethyl-group bound to the agarose whilst ANX Sepharose™ 4 Fast Flow (high sub) has a diethylaminopropyl group attached.
| TECHNICAL SPECIFICATIONS |
| Medium | Q Sepharose™ Fast Flow |
| | SP Sepharose™ Fast Flow |
| | CM Sepharose™ Fast Flow |
| | DEAE Sepharose™ Fast Flow |
| | ANX Sepharose™ 4 Fast Flow (high sub) |
| Column volume | 1 ml or 5 ml |
| Max. flow rate* |
| 1-ml column | 4 ml/min |
| 5-ml column | 20 ml/min |
| Recommended flow rate |
| 1-ml column | 1 ml/min |
| 5-ml column | 5 ml/min |
| Max back pressure | 3 bar (43 psi, 0.3 MPa) |
| Storage | 20% ethanol (Q, DEAE, ANX, CM), 0.2 M sodium acetate in 20% ethanol (SP) |
| Storage temperature | 4°C to 30°C |
| * H2O at 25°C
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