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GE Healthcare Life Sciences Part of GE Healthcare
  
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Last updated:07/31/2010

MiniBeads™ Columns

  • Innovative,monodisperse 3-μm nonporous beads yield exceptional resolution, speed, reproducibility, and durability with somewhat lower capacity than MonoBeads™.
  • Tricorn™ high-performance columns (4.6/50 PE) prepacked with MiniBeads™ are optimized for use with ÄKTAdesign and comparable LC systems.
  • Nonporous matrix of MiniBeads™ improves resolution and flow kinetics, better withstands back pressure, and minimizes nonspecific adsorption of the sample.
  • Maximum resolution is achieved from the efficiency of small, perfectly spherical monodispersed particles optimally packed in columns coupled with the excellent selectivities of the Q and S ion exchangers.
  • PC columns are designed for micropurification.
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*   Tricorn columns have Valco fittings for ÄKTAdesign systems and are supplied with M6 connectors for FPLC System. Tricorn columns are design patented, see back of catalog for details.
†   PC columns require the use of Precision Column Holder (see Precision Column Holder) when used with standard chromatography systems.
* Tricorn™ columns have Valco fittings for ÄKTAdesign™ systems and are supplied with M6 connectors for FPLC™ System. Tricorn™ columns and components are design patented.
† PC columns are designed for use with SMART systems. PC columns require the use of Precision Column Holder when used with chromatography systems other than SMART System.

MiniBeads™ Columns

Technical Information


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MiniBeads ion exchange media are available prepacked in Tricorn and PC columns for high-performance, high-resolution purification of proteins, peptides, and polynucleotides.

TECHNICAL SPECIFICATIONS
MiniBeads™
Ion exchanger type 
Mini Q™ Quaternary ammonium strong anion 
Mini S™ Methyl sulfonate strong cation 
Ionic capacity    
Mini Q™ 85-115 μmol Cl-/ml packed medium 
Mini S™ 13-29 μmol H+/ml packed medium 
pH stability 3-11 (working), 2-14 (short term) 
Chemical stability Stable in all common buffers except high concentration oxidizing and reactive agents, DMSO, DMF, and formic acid. 
Base matrix Monodisperse polystyrene/divinyl benzene particles 
Particle size 3 μm 
Storage 20% ethanol (Q), 0.2 M sodium acetate in 20% ethanol (S) 
Storage temperature 4°C to 30°C 
MiniBeads™ Columns*
Bed volume   
PC 3.2/3 0.24 ml 
4.6/50 PE 0.8 ml 
Max. pressure   
PC 3.2/3 100 bar (1450 psi, 10 MPa) 
4.6/50 PE 180 bar (2610 psi, 18 MPa) 
Recommended flow rate   
PC 3.2/3 0.1-1.0 ml/min 
  4.6/50 PE 0.5-2.0 ml/min 
* Columns not suitable for use with ÄKTAprime™ plus system.

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The exceptional resolution with Mini S 4.6/50 PE makes it a powerful tool for purity check of synthetic oligonucleotides.



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