| TECHNICAL SPECIFICATIONS |
| Matrix | Highly cross-linked agarose, 6% |
| Average particle size | 34 μm |
| Binding capacity‡ | At least 40 mg histidine-tagged protein/ml medium when charged with Ni2+ |
| Compatibility | Stable in all commonly used buffers |
| Chemical stability* | For one week at 40°C: 0.01 M HCl, 0.1 M NaOH
For 12 h: 1M NaOH, 70% acetic acid
30 min tested 30% 2-propanol
1 h tested 2% SDS |
| pH stability* | 3-12 (long term), 2-14 (short term) |
| HiTrap™ prepacked columns |
| Column volume | 1 ml and 5 ml |
| Column dimensions | 0.7 × 2.5 cm (1 ml), 1.6 × 2.5 cm (5 ml) |
| Recommended flow rate† | 1 ml/min (1 ml), 5 ml/min (5 ml) |
| Max. pressure† | 3 bar (0.3 MPa, 42 psi) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
* Medium uncharged with metal ions.
† H2O at room temperature.
‡ Protein- and metal-ion dependent.
|
Product related documents
Animation
<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
<sub>6</sub> from <italic>E. coli</italic> lysate using HiTrap IMAC HP column charged with different metal ions.'))
<sub>6</sub> fractions purified with different metal ions according to the chromatogram above. '))
