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HiLoad™ Phenyl Sepharose™ HP Columns and Phenyl Sepharose™ High Performance Lab Packs- Aromatic HIC medium for intermediate purification and polishing
- Selectivity similar to Phenyl Sepharose 6 Fast Flow (low sub) but higher resolution due to the smaller 34 μm bead size
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HiLoad™ Phenyl Sepharose™ HP Columns and Phenyl Sepharose™ High Performance Lab Packs
Technical Information
| TECHNICAL SPECIFICATIONS |
| Medium | Phenyl Sepharose™ High Performance |
| Ligand density | 25 μmol/mg medium |
| Binding capacity | 20 mg IgG/ml medium
24 mg HSA/ml medium |
| Average particle size | 34 μm |
| Rec. linear flow rate | 30-150 cm/h |
| Max. pressure | 5 bar (73 psi, 0.5 MPa) |
| Chemical stability | Stable in common buffers, chaotropic
agents, detergents, and polar
organic solvents. |
| pH stability | 3-12 (working and long term)
2-14 (short term) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
| Prepacked HiLoad™ Columns | |
| Bed volume | |
| HiLoad™ 16/10 | 20-22 ml |
| HiLoad™ 26/10 | 53-58 ml |
| Bed dimensions | |
| HiLoad™ 16/10 | 1.6 × 10 cm |
| HiLoad™ 26/10 | 2.6 × 10 cm |
| Efficiency | > 12 000 m-1 |
| Rec. flow rate | Up to 150 cm/h |
Max. back pressure over
the packed bed during operation | 3 bar (42 psi, 0.3 MPa) |
| HiLoad™ column hardware pressure limit | 5 bar (73 psi, 0.5 MPa) |
| Storage | 20% ethanol |
| Storage temperature | 4°C to 30°C |
Separation of conformational isomers of a2-macroglobulin on Phenyl Sepharose™ High Performance.
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