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Frequently Asked Questions

WAVE Bioreactor System

  • Single-use system means no cleaning, cross-contamination, or validation. Cells contact only disposable sterile biocompatible plastics.
  • Cellbag* based bioreactors, including all fittings and filters, are supplied sterile and ready for use. They are suitable for cGMP commercial production and a biosafety cabinet is not required for inoculation or sampling.
  • Multiple instrument configurations for suspension, microcarrier, batch, fed-batch, or perfusion culture.
  • Four different systems are capable of handling culture volumes from 50 ml to 500 l.
The WAVE Bioreactor is the ideal device for cell culture. Culture medium and cells contact only a presterile, disposable chamber called a Cellbag that is placed on a special rocking platform. The rocking motion of this platform induces waves in the culture fluid. These waves provide mixing and oxygen transfer, resulting in a perfect environment for cell growth that can easily support over 10 × 106 cells/ml. The WAVE Bioreactor requires no cleaning or sterilization, providing the ultimate ease in operation and protection against cross-contamination.

To select the correct system for your application, first determine the Cellbag size you need by establishing the culture volume you wish to cultivate and then select the instrumentation and options you need.

Applications

Monoclonal antibodies
The WAVE Bioreactor has been used extensively for monoclonal antibody production. Culture can be started at low volume and then fresh media added whenever the cell count is sufficiently high. This enables inoculum scaleup without transfers. Batches ranging from 100 ml to 580 l have been run with cell densities over 10 × 106 cells/ml and productivity and product quality comparable to stirred tank bioreactors. Dissolved oxygen concentrations are not limiting and remain above 50% saturation.

Anchorage-dependent cells
Agitation in the WAVE Bioreactor is powerful enough to mix and aerate the culture, yet it is gentle enough to cultivate anchorage-dependent cells on various microcarriers. The wave motion prevents settling and provides oxygenation without bubbles.

Virus production
The WAVE Bioreactor provides a closed system that is ideal for virus production. In a gene therapy application, human 293 cells have been grown in suspension and then infected with recombinant adenovirus. Cells grew to 4 × 106 cells/ml and virus production was 1 × 105 virus particles/cell. The WAVE Bioreactor produces viruses under complete containment without the need for a biosafety cabinet.

cGMP production
Wave Bioreactors are in use in cGMP applications producing inoculum for large conventional bioreactors, and also for clinical and commercial production of human therapeutics. Reduced cleaning and validation requirements make this an ideal system for cGMP applications.

Insect cell/baculovirus
The high oxygen supply capability of the WAVE Bioreactor makes it ideal for insect cell culture. Cell densities over 9 × 106 cells/ml are routinely achieved. Baculovirus yields are higher than with conventional bioreactors. The WAVE Bioreactor System is extremely easy to operate and inoculum scale-up and infection can be done inside the bioreactor, reducing the need for transfers.

Custom uses
The WAVE Bioreactor has many other uses, such as keeping in-process inoculum pools agitated and aerated prior to use; bead-to-bead transfer; thawing, and media mixing. Custom Cellbags can be provided for the WAVE Bioreactor for any working volume between 100 ml and 500 l.

* Cell Culture Bag in Switzerland.

SELECTION GUIDE - Systems
Culture volumeCellbagWAVE Bioreactor
50 to 250 mlCELLBAG-500 mlSystem 2/10
50 to 500 mlCELLBAG-1 lSystem 2/10
100 to 1000 mlCELLBAG-2 lSystem 2/10; System 20/50 + Kit 20
500 ml to 5 lCELLBAG-10 lSystem 2/10; System 20/50 + Kit 20
1 to 10 lCELLBAG-20 lSystem 20/50 + Kit 20
1 to 10 lCELLBAG-22 lSystem 20/50 + Kit 50
5 to 25 lCELLBAG-50 lSystem 20/50 + Kit 50
5 to 50 lCELLBAG-100 lSystem 200
10 to 100 lCELLBAG-200 lSystem 200
50 to 250 lCELLBAG-500 lSystem 500/1000 + Kit 500EH
100 to 500 lCELLBAG-1000 lSystem 500/1000 + Kit 1000EH

SELECTION GUIDE - Instrumentation Options
 Description
CO2 /Air Mix Plug-in ControllerInfrared CO2 sensor and aeration system provides a continuous supply of CO2 conditioned air to the Cellbag
Range: 0 to 15% CO2
Dissolved Oxygen Optical MonitorMonitor with miniature fiber-optic microprobes enabling real-time measurement of dissolved oxygen
High accuracy PMT optical detector with phase shift measurement
Range: 0 to 250% saturation with autozero
Reusable DOOPT-PROBE purchased separately
O2/Air Mix Plug-in ControllerProvides continuous supply of O2 enriched gas to the Cellbag for insect cell, virus, and high cell density applications.
Maintains low-oxygen environment for near-anaerobic applications
Range 0 to 50% O2
pH ControllerEnables continuous pH measurement and control using CO2 or acid/base addition.
Electrochemical probe is single use, and available pre-installed in Cellbags.
Loadcell2Enables online measurement of weight.
Used for automated fill/harvest and perfusion operations where precise volume control is critical.
Perfusion Controller3Perfusion Controller with Loadcell
1 Instrument Modules are available in WAVEPOD or as stand-alone units for System 2/10 and System 20/50. Fully integrated modules are available for System 200 and System 500/1000.
2 Fully integrated in System 20/50 (option); System 200 and System 500/1000 (standard).
3 For System 2/10 instrument only.

TECHNICAL SPECIFICATIONS - Systems
Unit  System 2/10 System 20/501 System 2002 System 500/10002,3 
Working volume range 
  • 0.1 to 5 l
 		0.1 to 25 l 5 to 100 l 50 to 500 l 
Integral features 
  • Speed/angle control
  • Temperature control
  • Aeration
 		Speed/angle control
Temperature control
Aeration 		Speed/angle control
Temperature control
Aeration
Loadcell 		Speed/angle control
Temperature control
Aeration
Loadcell
 
Options 
  • CO2MIX
  • O2MIX
  • DO
  • pH
  • Perfusion Controller
 
  • WAVEPOD
  • CO2MIX
  • O2MIX
  • DO
  • pH
  • Loadcell
  • Dual air/temperature
 
  • CO2MIX
  • O2MIX
  • DO
  • pH
  • Dual air/temperature
 
  • CO2MIX
  • O2MIX
  • DO
  • pH
 
Weight 
  • 4.2 kg
 
  • 15.5 kg
 
  • 350 kg
 
  • With Kit 500EH: 925 kg
  • with Kit 1000EH: 1020 kg
 
Dimensions 
  • 489 × 330 × 200 mm
 
  • 573 × 465 × 179 mm

  • with Kit 20:  711 × 575 × 254 mm
  • with Kit 50: 775 × 700 × 254 mm
 
  • 185 × 110 × 112 cm
  • For installation, if required, unit can be tilted
 
  • 201 × 124 × 160 cm
  • with Kit 500EH: 226 × 124 × 160 cm
  • with Kit 1000EH: 226 × 231 × 160 cm
 
Power 
  • 110/220 VAC
 
  • 100/240 VAC
  • 50/60 Hz, 6/3 A
 
  • 200 to 240 VAC
  • 50/60 Hz, 15 A
  • 3-Phase
  • Phase-phase ±5%
  • NEMA L2130 plug
 
  • 200 to 240 VAC
  • 50/60 Hz, 30 A
  • 3-Phase
  • Phase-phase ±5%
  • NEMA L2130 plug
 
1 System 20/50 requires selection of Kit 20 or Kit 50.
2 Unit provided with casters.
3 System 500/1000 requires selection of Kit 500EH or Kit 1000EH.
All BASE units are CE/CSA certified.



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