ÄKTA™ oligopilot™ plus


ÄKTA™ oligopilot™ plus is a flexible and fully automated DNA/RNA oligonucleotide synthesizer designed for use in research, process development laboratories, and production. ÄKTA oligopilot plus is designed to meet the needs of new synthesis chemistries like RNAi and enables cost-efficient and high-quality synthesis. ÄKTA oligopilot plus is a compact, pump-driven system employing flow-through reactor technology, which results in significantly reduced reagent consumption, enables exact control of reaction rates and contact times, and allows smooth scale up. ÄKTA oligopilot plus features column re-circulation, an important factor when performing RNA synthesis. ÄKTA oligopilot plus is available in two different configurations: ÄKTAoligopilot plus 10 for synthesis in the 1-50 µmol range and ÄKTAoligopilot plus 100 for synthesis in the 50 µmol-9 mmol range.
Features and benefits Flexibility in synthesis methods, chemistry, and scale. Cost-efficient and high-quality synthesis. Seven column positions and up to 12 monomer positions. Easy scale-up due to flow-through technology.
On-line monitoring with conductivity and UV-monitors. 21 CFR part 11 compatible UNICORN™ control software, as well as, Installation and Operational Qualification documentation are available.

Technical specification

Chemistry

Chemistry	Synthesis of DNA, RNA, phosphodiester, and phosphorothioat oligonucleotides using β-cyanoethyl, phosphoramidites.
Synthesis scales	1 µmol to 9 mmol depending on degree of substitution of solid support used.
Coupling efficiency	> 99% for DNA >98% for RNA
Cycle time	4.5 to 25 min for standard DNA
Reagent stability in instrument	At least 2 wk

Software

Controlled by UNICORN software on an external computer. The software is menu and mouse driven and allows method creation, synthesis system control, monitoring, and evaluation. UNICORN software is supplied on a CD. System-specific Strategy and Template methods are supplied on a separate CD.

Instrument

Dimensions: (W x H x D)	450 x 610 x 480 mm
Weight	63 kg
No. of column reactors	Up to seven
No of monomer ports	Eight fitted as standard. Option to use additional monomer inlets up to a total of 12.
Reagent delivery and washing	Active pumping with a flow rate range of 0.01 to 100 ml ÄKTA oligopilot plus 10: 2 x 10 ml/min ÄKTA oligopilot plus 100: 2 x 100 ml/min
Monitoring and control system	Conductivity monitoring. UV-vis monitoring of DMTr. Automatic calcuation of coupling efficiency. Pause capability if synthesis fails. Pressure.
Protective system	Argon or Nitrogen atmosphere
Delivery system	Eight low dead-volume motorized valves
Control system	UNICORN software on an external computer
Waste diversion system	Separates chlorinated from nonchlorinated hydrocarbons. Also possible to separate six other solvents, e.g monomer solutions.
Methods	Optimized, standard methods for synthesis in the range 1 μmol to 9 mmol. Customize methods by creating new methods or changing existing standard methods.
Warranty	One-year parts and labor

Literature

Application note

1-μmol synthesis of DNA using ÄKTA oligopilot plus 10 pdf

1-μmol synthesis of RNA using ÄKTA oligopilot plus 10 pdf

Optimization and scale-up of siRNA synthesis pdf

Robust and cost-efficient oligonucleotide synthesis pdf

Data file

ÄKTA oligo pilot pdf

Oligosynt prepacked disposable columns for the synthesis of long oligonucleotides pdf

Primer Support 200 for therapeutic oligonucleotide production pdf

User Manual

ÄKTA oligo pilot pdf

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