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Control software UNICORN

ÄKTApilot™

The sanitary system for rapid process development and small-scale production

ÄKTApilot™ is a high-performance, automated liquid chromatography system designed for process development, process scale-up, scale-down and small-scale production.The system has the capacity to purify from milligrams to tens of grams of product and is biocompatible, hygienic and sanitizable. ÄKTApilot meets all good laboratory practice GLP and current good manufacturing practice cGMP demands for Phase I–III in drug development and final-scale production.

Features and benefits

  • Hygienic design
    Enables purification of microbiologic- and contaminant-free products.
    For convenient Cleaning Validation.

  • High dynamic capacity
    Flow capacity 4-400 ml/min with 0-100% gradient.
    4-800 ml/min flow with limited gradient.
    Possible to purify 10 g product per cycle.
  • Fast and convenient start to every purification
    Method wizard for easy programming.
    Preprogrammed cleaning method and column lists.
    Pressure regulation of flow rate at sample applicationand column packing.

  • Validation support
    IQ/OQ documentation available.
    UNICORN™ control software supports FDA 21 CFR part 11 for Electronic signature and Electronic records.

  • Wetted parts easily changeable for
    Convenient product change when campaigning.
    Simplified cleaning validation.

  • Bench-top design
    Fits in small areas.
    Easily moved and serviced.

Technical specification

System pump and sample pump specifications

Flow rate
Isocratic and gradient mode
Isocratic and limited gradient

4–400 ml/min
Up to 800 ml/min
Pressure0–2 MPa (20 bar, 290 psi)
Flow rate accuracy< ±2% (4–400 ml/min, 0.1–2 MPa)
Flow rate reproducibilityrsd < 0.5% (4–400 ml/min, 0.1–2 MPa)
ViscosityMax. 5 cP
Gradient composition accuracy ±3% at 8–400 ml/min
Hold up volume615–1760 μl

UV flow cell specifications

Path length1 mm, 2 mm, 5 mm
Max. pressure2 MPa (20 bar, 290 psi)
Max. backpressure0.03 MPa (0.3 bar, 4.2 psi)
Wavelength accuarcy±2 nm
Wavelength precision±0.01 nm
Hold up volume300 μl

pH flow cell specifications

pH range0–14 (spec. valid between 2 and 12)
Response timeMax. 10 s (0–95% of step)
Max. pressure0.5 MPa (5 bar, 72 psi)
Max. backpressure0.01 MPa (0.1 bar, 1.4 psi)
Hold up volume240 μl

Membrane valve specifications

Max. pressure2.2 MPa (22 bar, 319 psi)
Max. backpressure0.015 MPa (0.15 bar, 2 psi)
pH stability range0–14 (spec. valid between 2–12)
Valve principle Stepper motor-controlled membranes
Hold up volume 145–354 μl

Membrane valve specifications

Max. pressure2.2 MPa (22 bar, 319 psi)
Max. backpressure0.015 MPa (0.15 bar, 2 psi)
pH stability range0–14 (spec. valid between 2–12)
Valve principle Stepper motor-controlled membranes
Hold up volume 145–354 μl

Membrane valve specifications

Max. pressure2.2 MPa (22 bar, 319 psi)
Max. backpressure0.015 MPa (0.15 bar, 2 psi)
pH stability range0–14 (spec. valid between 2–12)
Valve principle Stepper motor-controlled membranes
Hold up volume 145–354 μl

Conductivity cell specifications

Conductivity range1 μS/cm to 999.9 mS/cm
Deviation from theoretical Max. ±2% of full scale range or conductivity ±10 μS/cm whichever is greater in the range 1 μS/cm to 300 mS/cm
Response timeMax. 3 s (0–95% of step)
Temperature sensor

Accuracy
Drift



±2.0°C
±0.5°C per 10 h
Max. backpressure0.02 MPa (0.2 bar, 2.9 psi)
Hold up volume180 μl

Mixer specifications

Max. backpressure0.05 MPa (0.5 bar, 7 psi)
Mixing principleChamber with a stirrer
Hold up volume4.2 ml

Pressure sensor specifications

Max. pressure2.5 MPa (25 bar, 363 psi)
Max. backpressure0.06 MPa (0.6 bar, 9 psi)
Hold up volume 331 μl 2-port
346 μl 3-port

Literature

Data file

ÄKTApilot - The sanitary system for rapid process development and small-scale production pdf

Selection guide

Prepacked chromatography columns for ÄKTA™ design systems pdf

User Manual

ÄKTApilot pdf

Instrument Maintenance Agreements with Labcrew™

Ensure the maximum performance of your GE Healthcare Life Sciences instrument system with Service Agreements and Preventive Maintenance.A Service Agreement for your instument system helps to maintain long-term performance and to insure your investment against any unexpected breakdown costs. Learn more in the Service area.

Fast Trak™ Training & Education

Get the training you need in chromatography, process development, column packing, filtration or cell culture with Wave™. Fast Trak Training & Education has over 20 years of experience that provide you with the skills and knowledge for your industrial bioprocessing.

The Online Basic Training Course for UNICORN™ helps you exploit the capabilities of ÄKTA™ protein purification systems with ease and full control. Complete the training at your own pace. The course is structured in logical modules and is accessible for one year. Exercises are taken from ÄKTApurifier™ and ÄKTAexplorer™, although the course is still relevant for ÄKTApilot™, ÄKTAfplc™ and Ettan™LC users.

GE also provides training for a wide range of other application areas. Contact your local representative at GE Healthcare Life Sciences for further information.

Direct answers to laboratory and process-scale chromatography questions

Keep your research results coming in and your process operations on track. We've gathered our chromatography technical support on one single portal. The result will minimze the time you spend chasing answers to questions about spare parts and accessories, materials, packing and testing methods and many similar topics. Quickly find what you're looking for on Chromatography online support. On Chromatography online support you will find Spare parts and Accessories for ÄKTApilot.


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