ÄKTAprime™ plus - For simple purification of proteins


ÄKTAprime plus is a compact one-step purification system that operates with most prepacked columns. For reliability and convenience, we recommend HiTrap™, HiPrep™ or HiLoad™ columns that easily can be attached to the unit. The system has preprogrammed methods for typical applications such as purification of affinity tagged proteins, antibodies and for sample clean-up. The flexible system can be used for more general purification tasks such as buffer exchange, gel filtration, ion exchange, HIC, and affinity chromatography.
Features and benefits Simple to use - preprogrammed templates with optimized protocols for typical applications. Reliable results - automation removes the risk of errors, such as spillage or misplaced tubes, when doing manual work. Affinity protein purification - optimized push button methods together with prepacked HiTrap columns for maximum capacity. Know exactly where your protein is with PrimeView™- real time monitoring and guided evaluation.
Monitor and evaluation software PrimeView™ PrimeView allows the user of ÄKTAprime or ÄKTAPrime plus to view the monitor signals from the chromatographic run in real time on the computer screen. The Logbook pane displays all actions during a separation run, e.g. method start and end, base instruction, method instructions and manual instructions such as Pause or Hold. A result file is automatically generated at the end of a run and contains a complete record of the run, including method information, system settings, curve data, and a run log. The PrimeView Evaluation module provides extensive facilities to evaluate curve data and to generate a printed report from the stored result. Features and benefits Monitor a chromatographic run in real-time Easy to use chromatography data evaluation ÄKTAprime plus is connected to a PC via RS232 or a USB adapter

Technical specification

Size	400 x 450 x 530 mm (W × D × H)
Weight	13 kg
Solvent compatibility	All aqueous buffers commonly used in chromatography of biomolecules.

Pump

Flow rate range	0.1–50 ml/min
Increment	0.1 ml/min
Pressure range	0–1 MPa (10 bar, 145 psi)
Pressure limits	Programmable upper limit
Viscosity	Up to 10 cP

Monitor

Absorbance range	0.01–5 AU (full scale)
Wavelengths with Hg lamp (using filter selection)	254 , 280 nm (supplied) 313, 405, 436, 546 nm (optional)
With Zn lamp	214 nm
Linearity	<3% up to 2 AU at 254 nm <5% up to 1 AU at 280 nm
Noise	≤40× 10–6 AU
Drift	≤100× 10–4 AU
Maximum pressure	4 MPa (40 bar, 580 psi)
Flow rate	0–100 ml/min
Optical path length	2 mm

Conductivity measurement

Conductivity range

1 μS/cm–999.9 mS/cm (IEX and HIC gradients)

pH measurement (optional)

pH range	0–14 (specifications valid between 2–12)
Accuracy	± 0.1 pH unit, temperature compensated
Stability maximum	0.1 pH units deviation/10 h

Fraction collector (with flow diversion valve PSV-50)

Tube capacity

95 in Tube Rack 18 mm (supplied)
175 in Tube Rack 12 mm (optional)
40 in Tube Rack 30 mm (optional)

Environment

Ambient temperature	+4 – +40 °C
Relative humidity	10–95% relative humidity
Atmospheric pressure	84–106 kPa (840–1060 mbar)

Literature

Brochure

Protein purification - at the touch of a button pdf

Selection guide

Prepacked chromatography columns for ÄKTA™ design systems pdf

Software change description

PrimeView v 5.0 vs v 1.0 pdf

User Manual

PrimeView™ pdf

ÄKTAprime plus pdf

Instrument Maintenance Agreements with Labcrew™

Ensure the maximum performance of your GE Healthcare Life Sciences instrument system with Service Agreements and Preventive Maintenance.A Service Agreement for your instument system helps to maintain long-term performance and to insure your investment against any unexpected breakdown costs. Learn more in the Service area.

Fast Trak Training & Education

Get the training you need in chromatography, process development, column packing, filtration or cell culture with Wave™. Fast Trak Training & Education has over 20 years of experience that provide you with the skills and knowledge for your industrial bioprocessing.

GE also provides training for a wide range of other application areas. Contact your local representative at GE Healthcare Life Sciences for further information.

Direct answers to laboratory and process-scale chromatography questions

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