ÄKTApurifier™ Versatile FPLC purification of proteins and peptides

ÄKTApurifier, together with ÄKTAdesign automation kits, ensure that purification efforts are always focused on target proteins of interest. Purification can be performed at microgram, milligram or gram scale, depending on the system and kits selected. Optional ÄKTAdesign automation kits and hardware allow functionality to be added on when application needs change. By making a few simple choices at the beginning, you can find the ÄKTApurifier configuration that’s right for your lab. For purification of proteins at microgram and milligram scale, choose an ÄKTApurifier 10 system. Purification of proteins up to gram scale quantities of protein is easily achieved with any of the ÄKTApurifier 100 systems. There are three core ÄKTApurifier systems at each flow with essential functions for versatile FPLC. 1. ÄKTApurifier comes in standard configurations equipped with a triple wavelength UV monitor that allows detection of a wide range of proteins and contaminants. Together with the optional Monitor pH/C-900, temperature, pH and conductivity are measured in the flow path. 2. ÄKTApurifier plus is an extended version of ÄKTApurifier. With ÄKTApurifier plus, the most widely used automation kits are pre-installed and pre-tested, and you get hassle-free purification and increased automation right from the start. 3. ÄKTApurifier UPC systems offer robust monitoring of UV absorption at a single wavelength, as well as simultaneous conductivity and pH. These systems are economical alternatives and are designed for basic purification tasks. UNICORN™ protein purification software is used by more than 100,000 people worldwide involved with tasks ranging from research to manufacturing. Our software is recognized for its ability to generate high-quality purification results based on its flexible and intuitive method concept supported by advanced tools for result assessment.
ÄKTApurifier - Features and benefits Increased automation for convenient optimization of protein purification with extended configuration from the start with ÄKTApurifier plus Versatile system configurations with automation kits for all ÄKTApurifier systems Reproducible results Available with different pumps and monitors to fit a broad range of applications Fast and easy start to purification - UNICORN method wizard enables easy programming Design your own ÄKTApurifier system using our Configurator tool.

Overview ÄKTApurifier, options and technical data

= Standard feature
o =Optional feature
N/A= Not applicable

		ÄKTApurifier 100 plus	ÄKTApurifier 10 plus	ÄKTApurifier 100	ÄKTApurifier 10	ÄKTApurifier UPC 100	ÄKTApurifier UPC 10
ug – mg scale Gradient pump P-903, 10 ml/min, 250 bar (25 MPa))		N/A		N/A		N/A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
mg – gram scale Gradient pump P-901, 100 ml/min, 100 bar (10 MPa))			N/A		N/A		N/A
------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Control and Detection
Reliable system control software UNICORN
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Triple wavelength detection, 190-700 nm - Monitor UV-900						N/A	N/A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
pH, conductivity, and temperature monitor - pH/C-900				o	o	N/A	N/A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Single-wavelength UV, pH and conductivity Combi-monitor UPC-900		N/A	N/A	N/A	N/A
------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Add Automation
Simple large size fractionation Fractionation Kit				o	o	N/A	N/A
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Automated application of multiple samples Sample application Kit			o	o	o	o	o
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Automated column scouting Column Screening kit				o	o	o	o
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Optimized flow path from the start - Preinstalled capillaries				o	o	o	o
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
On-line buffer preparation and buffer selection - BufferPrep Kit				o	o	o	o
------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Add Versatility
Air protection Air sensor Kit		o	o	o	o	o	o
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Sharper peak elution Reverse flow Kit		o	o	o	o	o	o
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Versatile buffer selection Buffer selection Kit		o	o	o	o	o	o
------------------------------------------------------------------------------------------------------------------------------------------------------------------------- Add Sample collection and Fractionation
Simple and robust fraction collection Fraction collector Frac-920		o	o	o	o	o	o
------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Versatile fraction collection Fraction collector Frac-950		o	o	o	o	o	o
------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Automated application of multiple, cooled samples Autosampler A-905		o	o	o	o	o	o
------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Dimensions, mm (W x D x H)		500 x 460 x 620
------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Weight (kg)		75
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Solvent compatibility	All commonly used chromatographic solvents
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Operating temperature (oC)		4-40
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Voltage	100-120/220-240 VAC, 50-60 Hz
-------------------------------------------------------------------------------------------------------------------------------------------------------------------------
Safety certifications	CE 89/336/EEC (EMC directive), CE 73/23/EEC (LV directive), EN-61010-1 (IEC1010-1, UL3101, CSA22.2 1010-1)

Literature

Brochure

What sort of purifier are you? There’s an ÄKTApurifier that matches your needs pdf

Data file

ÄKTApurifier pdf

Selection guide

Prepacked chromatography columns for ÄKTA™ design systems pdf

User Manual

ÄKTApurifier Getting started pdf

Instrument Maintenance Agreements with Labcrew™

Ensure the maximum performance of your GE Healthcare Life Sciences instrument system with Service Agreements and Preventive Maintenance. A Service Agreement for your instrument system helps to maintain long-term performance and to insure your investment against any unexpected breakdown costs. Learn more in the Service area.

Fast Trak Training & Education

The Online Basic Training Course for UNICORN™ helps you exploit the capabilities of ÄKTA™ protein purification systems with ease and full control. Complete the training at your own pace. The course is structured in logical modules and is accessible for one year. Exercises are taken from ÄKTApurifier™ and ÄKTAexplorer™, although the course is still relevant for ÄKTApilot™, ÄKTAFPLC™ and Ettan™LC users.

Get the training you need in chromatography, process development, column packing, filtration or cell culture with Wave™. Fast Trak Training & Education has over 20 years of experience that provide you with the skills and knowledge for your industrial bioprocessing.

GE also provides training for a wide range of other application areas. Contact your local representative at GE Healthcare Life Sciences for further information.

Direct answers to laboratory and process-scale chromatography questions

Keep your research results coming in and your process operations on track. We've gathered our chromatography technical support on one single portal. The result will minimize the time you spend chasing answers to questions about spare parts and accessories, materials, packing and testing methods and many similar topics. Quickly find what you're looking for on Chromatography online support. On Chromatography online support you will find Spare parts and Accessories for ÄKTApurifier.