ÄKTAxpress™ - A complete solution for unattended, multistep protein purification
ÄKTAxpress for tagged protein and antibody purifications gives you the highest possible purity and allows, for example, structural and functional studies to be easily performed. Optimized protocols with a choice of up to four purification steps minimizes the need for chromatography expertise. Tag removal and maintenance procedures can be integrated into the purification protocols, eliminating manual interference during a run. Purification schemes for antibodies, single and double affinity-tagged proteins are supported. A four-step protocol may consist of affinity chromatography (AC), desalting (DS), ion exchange chromatography (IEX) and gel filtration (GF). Desalting in-between different purification steps are optional UNICORN protein purification software is used by more than 50,000 people in more than a thousand manufacturing installations worldwide. Our software is able to generate high-quality purification results due to its flexible and intuitive method concept supported by advanced tools for result assessment.
ÄKTAxpress - Features and benefits Throughput This easy-to-use system allows purification of up to 4 different samples per system depending on used protocol. Capacity Get up to 50 mg amounts of pure target protein from each start sample. Purity The system delivers proteins of >95% purity depending on selected protocol and columns. Intelligence Purpose-designed hardware, easy-to-use software wizards and prepacked media deliver pure proteins in an automatic way. No chromatography expertise is required. The pre-optimized protocols gives you robust results.

Technical specification

System specification*

System flow rate	0.1-65 ml/min
Operating pressure	3 MPa (30 bar, 435 psi)
UV wavelength range	280 or 254 nm
Conductivity range	1 uS/cm to 999.9 mS/cm
Size	250 x 650 x 700 mm (W × D × H)
Weight	27 kg
Solvent compatibility	All aqueous buffers commonly used in chromatography of biomolecules, pH 0-14.

*The specifications are valid when working within the pH range of 2 to 12.

System pump and sample pump specifications

Flow rate	0.1-65 ml/min
Pressure	0-3 MPa (30 bar, 435 psi)
Flow rate accuracy	± 2%
Flow rate reproducibility	rsd < 0.5%

UV flow cell specifications

Path length	2 mm
Internal volume	30 µl
Max. pressure	4 MPa (40 bar, 580 psi)

Conductivity cell specifications

Conductivity range	1 μS/cm to 999.9 mS/cm
Deviation from theoretical	Max. ± 3% of full scale calibrated conductivity range or ± 15 μS/cm
Response time	Max. 3 s (0% to 95% of step)
Max. flow rate	100 ml/min
Max. pressure	5 MPa (50 bar, 725 psi)
Internal volume	24 µl

Mixer specifications

Max. flow rate	65 ml/min
Max. pressure	3 MPa
Mixing volume	0.37 ml

Literature

Application note

Automated multistep purification of (His)6 and GST-tagged proteins pdf

Automated multistep purification of tagged proteins for high-throughput structural studies pdf

Automated multistep purification of histidine-tagged proteins from unclarified cell lysates pdf

Automated on-column tag cleavage and multistep purification of (His)6 and GST-Tagged proteins pdf

Automated purification of dual-tagged proteins pdf

Automated purification and quantitation of soluble histidine-tagged integrase of Tn21 from E. coli cells
predominantly expressing the protein as inclusion bodies pdf

His MultiTrap FF and His MultiTrap HP prepacked 96-well filter plates - for reproducible purification
screening of histidine-tagged proteins pdf

High-throughput purification of antibodies pdf

New prepacked 96-well filter plates, His MultiTrap and GST MultiTrap, for purification screening of
Histidine and GST-tagged proteins pdf

Optimizing protocols for automated multistep purification of (histidine)6-tagged protein using ÄKTAxpress pdf

Brochure

ÄKTAxpress - Take advantage of your routines; be in two places at once pdf

Data file

ÄKTAxpress for tagged proteins pdf
ÄKTAxpress MAb for antibody purification pdf

Selection guide

Prepacked chromatography columns for ÄKTA™ design systems pdf

User Manual

ÄKTAxpress User manual pdf
ÄKTAxpress Cue cards pdf
ÄKTAxpress MAb User manual pdf
ÄKTAxpress MAb Cue cards pdf

Instrument Maintenance Agreements with Labcrew™

Ensure the maximum performance of your GE Healthcare Life Sciences instrument system with Service Agreements and Preventive Maintenance.A Service Agreement for your instument system helps to maintain long-term performance and to insure your investment against any unexpected breakdown costs. Learn more in the Service area.

Fast Trak Training & Education

Get the training you need in chromatography, process development, column packing, filtration or cell culture with Wave™. Fast Trak Training & Education has over 20 years of experience that provide you with the skills and knowledge for your industrial bioprocessing.

GE also provides training for a wide range of other application areas. Contact your local representative at GE Healthcare Life Sciences for further information.

Direct answers to laboratory and process-scale chromatography questions

Keep your research results coming in and your process operations on track. We've gathered our chromatography technical support on one single portal. The result will minimze the time you spend chasing answers to questions about spare parts and accessories, materials, packing and testing methods and many similar topics. Quickly find what you're looking for on Chromatography online support. On Chromatography online support you will find Spare parts and Accessories for ÄKTAxpress.