KappaSelect

KappaSelect is an affinity chromatography medium designed for the purification of Fab (kappa) with high binding capacity, purity and yield
Rigid agarose base matrix allows high flow rates and processing of large sample volumes for increased throughput
Non-mammalian derived product reduces regulatory concerns in the production of Fabs for clinical applications

KappaSelect

Technical Information

Antibody fragments, especially Fabs, are getting increased attention as potential biopharmaceuticals because they have some advantages over monoclonal antibodies (Mabs). For example, Fabs show improved pharmacokinetics for tissue penetration and can bind to targets inaccessible to conventional antigen-binding sites.

KappaSelect is an affinity chromatography medium designed for the purification of Fab (kappa) antibody fragments. The ligand binds to the constant region of the kappa light chain enabling an efficient capture step for antibody fragments such as Fabs with high purity and yield. KappaSelect is part of GE Healthcare's Custom Designed Media program.

TECHNICAL SPECIFICATIONS
Composition	Highly cross-linked high-flow agarose
Average Particle size	75 µm (d₅₀, vol)
Ligand	Recombinant protein (Mr 13 000), produced in S. cerevisiae, with affinity for the constant domain of the immunoglobulin kappa light chain
pH stability (operational)	3-10
CIP stability (short term)	2-12
Pressure/flow spec	At least 600 cm/h in a 1 m column with 20 cm bed height at 20°C using process buffers with the same viscosity as water at < 3bar (0.3 Mpa)
This product is part of our Custom Designed Media program and not yet a standard product. If you are interested in large-scale quantities, please contact your local GE Healthcare representative