Discovery Matters 14

• Multistep purification of humanized WO-2 Fab for crystallization studies using ÄKTAxpress
• Multistep purification of recombinant tagged proteins for SPR studies using ÄKTAxpress
• The use of high-content analysis to develop a high-throughput wound healing assay for cancer research
• Simplified manual purification of antibodies in 30 min
• Comparative performance of blue film for visualization of proteins
• Details of our latest products and services

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Discovery Matters 13

• Stability of membrane proteins analyzed by gel filtration
• Screening of extraction buffers for 2-D DIGE analysis of plant proteins
• A benchmark study of streptavidin magnetic beads
• Rapid sample preparation for MicroCal™ ITC and DSC experiments using PD MidiTrap™ G-25 columns
• Concentration and recovery of proteins from cell culture media using Vivaspin sample concentrators
• High-content analysis of a live multiplexed cytotoxicity study using Cytiva™ Cardiomyocytes and IN Cell Analyzer 2000
• Cancer research tools from GE Healthcare

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Discovery Matters 12

• Inaugural winner of the GE Healthcare High-Content Analysis (HCA) Award
• High-content analysis using IN Cell Analyzer 1000 permits dissection of diverse signaling pathways for T lymphocyte polarization
• Outstanding sensitivity for confident label-free interaction analysis using Biacore T200
• Increased sensitivity and stability in Western blotting using Amersham ECL Prime
• Rapid buffer scouting and quality control of integral membrane proteins
• High-throughput screening for antibodies and histidine-tagged proteins using Mag Sepharose magnetic beads
• Increasing the yield of purified GST-tagged proteins

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Discovery Matters 11

• Synchronized solutions for electrophoresis
• Matrix-assisted refolding of histidine-tagged proteins
• Sensitive labeling for array CGH
• Protein binding studies using label-free interaction analysis
• High-content assays using IN Cell Analyzer 2000

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Discovery Matters 10

• Fast and secure method/process development with new ÄKTA™ avant 25 and UNICORN™ 6
• Short gel filtration columns for rapid purity analysis of a tagged recombinant protein One-step purification of Mg-chelatase subunit BchI using HisTrap™ FF crude
• Micropreparative purification of PEP-19 protein from low amounts of mouse brain tissue using ÄKTAmicro™ chromatography system
• Cultivation of adherent cells on Cytodex™ microcarriers using WAVE Bioreactor™ Systems 2/10 and 20/50

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Discovery Matters 9

• A simple, two-step purification method designed forpurification of histidine-tagged membrane proteins
• Convenient and efficient methods for cell lysis and protein extraction
• Convenient and rapid concentration of protein solutions for X-ray crystallography using Vivaspin sample concentrators
• Reliable detection and characterization of anti-drug antibodies using Biacore T100 Immunogenicity Package
• Comparative analysis of illustra triplePrep Kit for the isolation of genomic DNA, total RNA, and total denatured protein from a single undivided sample
• Understanding a minimal DNA-segregating machine - Reconstitution of a plasmid spindle shows how three components together accomplish the task of DNA segregation

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Discovery Matters 8

• Efficient sample cleanup of low molecular weight carbohydrates
• Desalting, buffer exchange and concentration of proteins,peptides, and carbohydrates
• Drug screening and cellular assays Imaging promyelocytic leukemia nuclear bodies using IN Cell Analyzer 1000
• Automation of high-throughput expression screening on His MultiTrap HP with vacuum elution using a Hamilton Liquid Handling Workstation
• HiScreen columns for process development
• Preparation of genomic DNA from dermatophytes (cultures and clinical samples) using illustra tissue & cells genomicPrep Mini Spin Kit

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Discovery Matters 7

• Simplify the process of DNA isolation with illustra GenomiPhi & TempliPhi
• Faster preparation of histidine-tagged proteins with His SpinTrap
• Enrichment of phosphorylated peptides using Phos SpinTrap Fe improves protein identification by mass spectrometry
• Fast buffer scouting for membrane protein purification using small-volume gel filtration
• High-throughput expression screening of histidine-tagged proteins
• Biacore A100 for compound screening and affinity ranking in fragment-based drug design
• Cell Factory Service: the bulk provision of receptor membranes for high-throughput screening
• Nucleic acid synthesis, amplification, and analysis Amplification of DNA from diverse sources with the illustra GenomiPhi HY DNA Amplification Kit 30
• New ozone and photostable HyPer5 fluorescent dye for microarray applications
• Rapid production of clinical grade T lymphocytes

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Discovery Matters 6

• Genomic DNA preparation using illustra GenomiPhi V2 and HY DNA Amplification Kits
• Amersham CyScrisbe Array CGH Genomic DNA Labeling System identifies chromosomal abnormalities in prostate cance
• Multiplex assays using Ad-A-Gene Vectors: EGFP-GCC R(EGFP-glucocorticoid receptor) and GRE-NTR (glucocorticoid response element-nitroreductase)
• Multiplex 2-D Western blotting and internal standardization: novel applications of the Amersham ECL Plex system
• 2-D DIGE enables detection of an APOE-regulated protein the hippocampus of Alzheimer’s disease patients1
• Chromatographic analysis of glycoproteins and their deglycosylated forms
• Low-cost, automated serial purification of histidinetagged proteins
• Easy purification of GST-tagged proteins

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Discovery Matters 5

• Optimization of assay parameters for the Ad-A-Gene EGFP-MAPKAP kinase 2 assay
• Monitoring expression of an E. coli membrane protein GFP-(His)8 fusion and purification using a combination of IMAC and gel filtration
• Purification of phosphorylated protein kinases using MonoBeads ion exchange media
• Parallel, affinity-based sample preparation using Protein G HP MultiTrap to speed up LC-MS protein analysis
• Rapid purification of high-quality plasmid DNA using illustra plasmidPrep Mini Spin Kit
• Optimization of CypHer5E labeled anti-VSV-G antibody for use in receptor internalization assays

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Discovery Matters 4

• Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
• Detection, identification, and quantitation of an olive allergen using Ettan MDLC, MS/MS, and DeCyder MS
• LC-MALDI target spotting and microfraction collection with nanoCOLLECT
• Increased PCR amplification specificity using illustra Hot Start Master Mix
• Two-step, automated purification of GST-hippocalcin using GSTrap 4B and HiLoad 16/60 Superdex 200 pg columns on ÄKTAxpress chromatography system
• In depth analysis of multiparameter high-content data using Spotfire DecisionSite software
• Nucleic acid blotting without radioactivity using Amersham AlkPhos Direct

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Discovery Matters 3

• Analysis of tomato fruit ripening with DeCyder EDA software
• Automated processing of cord blood
• Microgenomics: gene expression profiling of laser-capture microdissected cells
• Comparing Ad-A-Gene adenoviral transduction with plasmid based transfection
• Purification of histidine-tagged proteins using various metal ions
• Process development using His MultiTrap FF 96-well filter plates on ÄKTAxpress
• Solutions for histidine-tagged protein purification
• Validation of Redivue Tip [γ-32P] ATP in a Biotrak PKC enzyme assay
• High-throughput screening using CypHer5E and IN Cell Analyzer 1000

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Discovery Matters 2

• Homogeneous, chemiluminescent kinase assay kits for assessing the functional activity of a broad range of serine-threonine and tyrosine kinases
• Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
• GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
• Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
• A sensitive microgel filtration method to analyze the lipoprotein profile of individual serum samples on a ÄKTAbasic 10 chromatography system
• ÄKTAcrossflow—the intelligent membrane filtration system
• Addition of imidazole during binding improves purity of histidine-tagged proteins
• New gravity-flow column gives faster and simpler purification of histidine-tagged proteins
• Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting
• Noise level comparison of gel filtration columns with inline light-scattering detection on ÄKTAdesign system
• Measurement of pollen associated lipid mediators with structural and functional similarities to eicosanoids

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Discovery Matters 1

• siRNA screening of the cell cycle with two dynamic GFP sensors
• SPA Imaging Charge-Based Binding Beads: A different approach to the capture of assay product
• Resolving truncated forms of histidine-tagged proteins
• A demonstration of tandem affinity purification (TAP)
• Automated protein data analysis for LC-MS/MS
• Comparing media for analytical gel filtration
• Staining blots with Deep Purple Total Protein Stain
• Selective labeling of cell surface proteins
• Gravity-flow purification of histidine-tagged proteins
• Creating a shotgun library from glycerol stocks without culturing
• Generating micrograms of plant DNA from small samples
• Oligonucleotide probes for validating microarray data
  

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